Localisation

Can anyone tell me an advantage and a disadvantage of over expressing proteins in mammalian cells to discover their function or localisation?

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PINs are transporters of auxin which are localised on one side of cell forming polar auxin transport. And their localisation is driven by several factors... hmm what happens if auxin promotes apical dominance, then it cant grow anymore due to the limiting space?

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PINs are transporters of auxin which are localised on one side of cell forming polar auxin transport. And their localisation is driven by several factors...

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but that's what the predictors do, they look on the primary structure and look for specific patterns, which could mean localisation into any organelle

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mutate the gene X to either silence or constitutively express and watch the localisation of your protein. Or just induce in some way

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