Dictionary » L » Loading

Loading

Loading

administration of a substance for the purpose of testing metabolic function.


Please contribute to this project, if you have more information about this term feel free to edit this page



Results from our forum


Trying to prove height increase...need help

... bones just more force/pressure may be needed. I am not quite sure why it works but the method is based on Lengthening of Mouse Hindlimbs by Joint Loading by Ping Zhang/Hiroki Yokota. However, I had to use much more load than is present in the study(fairly intense clamping). Possible reasons could ...

See entire post
by MiniGolfer
Thu Jul 24, 2014 9:09 pm
 
Forum: General Discussion
Topic: Trying to prove height increase...need help
Replies: 2
Views: 255

electrophoretic mobility shift assay (EMSA)

... have a good tip what went wrong? (I use 6% non-danaturing PAA gel without a stacking gel, I pre-run it at 100V for 1h, I rinse the wells before loading, I don't vortex the samples, the loading buffer doesn't contain SDS either)

See entire post
by kk
Fri Nov 08, 2013 12:21 pm
 
Forum: Molecular Biology
Topic: electrophoretic mobility shift assay (EMSA)
Replies: 1
Views: 1144

Weird - shaped band on western blotting

... regulation protein. Recently I got a weird-shaped band on my western blot. It is like an upside-down of smiling face in each lane. I use the same loading buffer, electrophoresis buffer, lysis buffer, and also the same 1st and 2nd antibody for this experiment. I also try to reduce the amount of ...

See entire post
by namakulaila
Thu Oct 17, 2013 6:17 am
 
Forum: Molecular Biology
Topic: Weird - shaped band on western blotting
Replies: 2
Views: 1306

calcium measurement- help!!

... know exactly how to do it: what conc. should I use for Ionomycin? and EGTA? and when does the EGTA should be added? before, during or after the loading? with the stimulant? after it? thanks in advance! :D Ruth

See entire post
by reulita10
Tue Jul 16, 2013 12:35 pm
 
Forum: Molecular Biology
Topic: calcium measurement- help!!
Replies: 0
Views: 450

calcium measurement- help!!

... know exactly how to do it: what conc. should I use for Ionomycin? and EGTA? and when does the EGTA should be added? before, during or after the loading? with the stimulant? after it? thanks in advance! Ruth

See entire post
by reulita10
Tue Jul 16, 2013 12:34 pm
 
Forum: General Discussion
Topic: calcium measurement- help!!
Replies: 0
Views: 455
View all matching forum results

This page was last modified 21:16, 3 October 2005. This page has been accessed 1,728 times. 
What links here | Related changes | Permanent link