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administration of a substance for the purpose of testing metabolic function.


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electrophoretic mobility shift assay (EMSA)

... have a good tip what went wrong? (I use 6% non-danaturing PAA gel without a stacking gel, I pre-run it at 100V for 1h, I rinse the wells before loading, I don't vortex the samples, the loading buffer doesn't contain SDS either)

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by kk
Fri Nov 08, 2013 12:21 pm
 
Forum: Molecular Biology
Topic: electrophoretic mobility shift assay (EMSA)
Replies: 1
Views: 645

Weird - shaped band on western blotting

... regulation protein. Recently I got a weird-shaped band on my western blot. It is like an upside-down of smiling face in each lane. I use the same loading buffer, electrophoresis buffer, lysis buffer, and also the same 1st and 2nd antibody for this experiment. I also try to reduce the amount of ...

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by namakulaila
Thu Oct 17, 2013 6:17 am
 
Forum: Molecular Biology
Topic: Weird - shaped band on western blotting
Replies: 2
Views: 831

calcium measurement- help!!

... know exactly how to do it: what conc. should I use for Ionomycin? and EGTA? and when does the EGTA should be added? before, during or after the loading? with the stimulant? after it? thanks in advance! :D Ruth

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by reulita10
Tue Jul 16, 2013 12:35 pm
 
Forum: Molecular Biology
Topic: calcium measurement- help!!
Replies: 0
Views: 337

calcium measurement- help!!

... know exactly how to do it: what conc. should I use for Ionomycin? and EGTA? and when does the EGTA should be added? before, during or after the loading? with the stimulant? after it? thanks in advance! Ruth

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by reulita10
Tue Jul 16, 2013 12:34 pm
 
Forum: General Discussion
Topic: calcium measurement- help!!
Replies: 0
Views: 337

How can i asses the parasie loading of littorina littoria?

There is too much variation in the parasite loading of gastropods.

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by marineman
Sat Mar 02, 2013 10:27 am
 
Forum: Zoology Discussion
Topic: How can i asses the parasie loading of littorina littoria?
Replies: 7
Views: 2731
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