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administration of a substance for the purpose of testing metabolic function.

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separation problem with GelRed

... in prestain gels, leading to some separation problems. Here are some solutions to improve band separation: 1. Reduce the amount of loaded DNA. Overloading of DNA often results in band smearing. The recommended amount for ladders and samples of known concentration is 50-200 ng/lane. If the concentration ...

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by Biotium
Wed Nov 26, 2014 4:43 pm
Forum: Molecular Biology
Topic: separation problem with GelRed
Replies: 12
Views: 19559

Trying to prove height increase...need help

... bones just more force/pressure may be needed. I am not quite sure why it works but the method is based on Lengthening of Mouse Hindlimbs by Joint Loading by Ping Zhang/Hiroki Yokota. However, I had to use much more load than is present in the study(fairly intense clamping). Possible reasons could ...

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by MiniGolfer
Thu Jul 24, 2014 9:09 pm
Forum: General Discussion
Topic: Trying to prove height increase...need help
Replies: 2
Views: 2551

electrophoretic mobility shift assay (EMSA)

... have a good tip what went wrong? (I use 6% non-danaturing PAA gel without a stacking gel, I pre-run it at 100V for 1h, I rinse the wells before loading, I don't vortex the samples, the loading buffer doesn't contain SDS either)

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by kk
Fri Nov 08, 2013 12:21 pm
Forum: Molecular Biology
Topic: electrophoretic mobility shift assay (EMSA)
Replies: 1
Views: 2005

Weird - shaped band on western blotting

... regulation protein. Recently I got a weird-shaped band on my western blot. It is like an upside-down of smiling face in each lane. I use the same loading buffer, electrophoresis buffer, lysis buffer, and also the same 1st and 2nd antibody for this experiment. I also try to reduce the amount of ...

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by namakulaila
Thu Oct 17, 2013 6:17 am
Forum: Molecular Biology
Topic: Weird - shaped band on western blotting
Replies: 2
Views: 2445

calcium measurement- help!!

... know exactly how to do it: what conc. should I use for Ionomycin? and EGTA? and when does the EGTA should be added? before, during or after the loading? with the stimulant? after it? thanks in advance! :D Ruth

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by reulita10
Tue Jul 16, 2013 12:35 pm
Forum: Molecular Biology
Topic: calcium measurement- help!!
Replies: 0
Views: 703
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