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... Ponceau's solution, i am getting distinct bands at higher molecular weight region but not getting anything below 35 kD region. i'm approximately loading 25 micro gram of protein. i have confirmed the observation by immunobloting the membrane with higher (VDAC-1) and lower molecular weight (COX-IV) ...

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... Then I loaded 2mL of the lysate to 3mL 35%sucrose cushion with dropper. I found that some drops distrubed the sucrose layer surface during the loading process. However, I found that there was still two liquid layers so that I continued the experiment. After 1hr centrifugation at 100000g in ...

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... Then I loaded 2mL of the lysate to 3mL 35%sucrose cushion with dropper. I found that some drops distrubed the sucrose layer surface during the loading process. However, I found that there was still two liquid layers so that I continued the experiment. After 1hr centrifugation at 100000g in ...

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... on... except that I can't retrieve my protein. Any advice you can offer on solution concentrations, pH... anything. Side note: When I add SDS loading buffer to the eluted samples a lot of white precipitate falls out... I've just been heating the sample for 10 min at 100degrees C and then spinning ...

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... as PaO2 levels fall b/c of a greater diffusibility of CO2 . Does it have to do w/ the different partial pressure gradients of the two gases when loading/unloading at the tissues? I've got myself all wound up here. There is a difference in the way both gases travel in the blood. The vast majority ...

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