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(Science: abbreviation) long interspersed elements.

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mycoplasma testing

... to start. We were thinking of using PCR based method as aprimary method and vero cells staining as a second method. not sure how often and what lines I need to test (we have fibroblasts and neuronal cultures). does anyone have a example of a protocol that is used in his lab of what specimens ...

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by revi
Tue Feb 25, 2014 11:03 am
Forum: Molecular Biology
Topic: mycoplasma testing
Replies: 0
Views: 107

Re: What do you do when talking to creationists?

... Indeed, will science add indefinitely without understanding completely? Anyway, is it possible to understand something completely? Along these lines, there is a different book, a preview in http://goo.gl/rfVqw6 Just another suggestion in order to freethink for a while

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by editorb
Sat Jan 18, 2014 2:33 pm
Forum: Evolution
Topic: What do you do when talking to creationists?
Replies: 4
Views: 1484

Re: Cell culture - no CO2

Wow! this was so so helpful. I have been wondering why I use non-filter flasks for GMK and Vero and filter for all mammalian cell lines we grow in my Lab. I love you guys. Thanx alot.

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by cheryl4ryl
Fri Nov 22, 2013 12:35 pm
Forum: Cell Biology
Topic: Cell culture - no CO2
Replies: 10
Views: 17993

Need an help about Drosophila genetics

Hello! I need help. My professor asks me this and I could not understand it. Can you please help me to figure it out? I asked him after crossing lines with UAS-UPRT and Gal-4 driver, how are we going to select the flies? He gave me this: Flies have 4 chromosomes: 2 and 3 are useful for genetics. ...

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by golgicik
Wed Oct 30, 2013 8:46 pm
Forum: Genetics
Topic: Need an help about Drosophila genetics
Replies: 5
Views: 1322

Passage number

Hi, There are different opinions about this but primarily it depends on the cells that you are growing. In differentiated cell lines the cells wont get reclocked at all. In the lab I work in, we dont consider that the cells can be reclocked. The best way of labelling would be labeling ...

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by jwalin
Fri Aug 30, 2013 2:26 pm
Forum: Cell Biology
Topic: Passage number
Replies: 1
Views: 566
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