Dictionary » L » Linear



Pertaining to or resembling a line.

Origin: L. Linearis

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Minimum Data Points for elisa standard curve

... for only 5 data points( 5 standards)? 2. In Elisa test instruction that the company gave me, it is noted that the calibration curve should be a linear plot. But my results can be better explained by non linear curves. Am I allowed to disregard test instruction in this case? Any help would be ...

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by rgm
Sun Feb 01, 2015 7:19 am
Forum: Molecular Biology
Topic: Minimum Data Points for elisa standard curve
Replies: 0
Views: 327

holographic origin of life

... and we give the exact food, then if these organisms are just random chemical reactions, their lifespan could be predicted as a result of multiple linear regression. The dependent variable y (or else the lifespan) would be: y=a+a1x1+a2x2+…….aνxν+aωxω+ε where ε is the error variable and x1,x2…xν ...

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by minasole
Wed Aug 28, 2013 5:15 pm
Forum: Evolution
Topic: holographic origin of life
Replies: 15
Views: 27488

Problem with the normalization of PCR Ct values

You should not divide the CTs, because they are not linear. You have to subtract them and the difference will tell you how many times more copies there was.

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by JackBean
Tue Apr 30, 2013 3:55 pm
Forum: Molecular Biology
Topic: Problem with the normalization of PCR Ct values
Replies: 5
Views: 8320

Western Blot

... is, otherwise known as its molecular weight. When extracts are prepared from a cell, detergents can be used to make the proteins unfold into the linear chain of amino acids. Using a detergent such as sodium dodecyl sulfate (SDS) makes proteins unfold and gives them a net negative charge that ...

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by MarkHolland
Sat Mar 30, 2013 3:22 pm
Forum: Cell Biology
Topic: Western Blot
Replies: 6
Views: 4464

SDS-PAGE gel and Rf values

... weight markers in lane one of the gel that I attached. When I do this and graph the Rf values against the molecular weight my graph is not linear. I marked in red where I am measuring from. Can someone please tell me what I am doing wrong?

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by cschauss
Sun Mar 03, 2013 7:37 am
Forum: Cell Biology
Topic: SDS-PAGE gel and Rf values
Replies: 2
Views: 3382
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