Dictionary » L » Linear

Linear

Linear

Pertaining to or resembling a line.

Origin: L. Linearis


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Protein structure

... primary,secondary, Tertiary and Quaternary so far this is what i understand by the following terms ok so far i know the primary structure is linear arrange of the amino acids in a peptide chain to show the sequence and number of amino acids in the chain The secondary structure is the arrangement ...

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by ccBiologist
Thu Sep 29, 2011 8:15 am
 
Forum: Molecular Biology
Topic: Protein structure
Replies: 2
Views: 195

Re: Relationship between structure and function of glucoes

... have different mechanical properties and adopt different confirmations, with poly-α-D-glucose forming curving fibers and poly-β-D-glucose forming linear fibers (as you wrote, Adz, due to different hydrogen-bonding geometries). I don't think there is a similar logic to the L- vs. D- amino acids. ...

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by jonmoulton
Tue Sep 13, 2011 3:48 pm
 
Forum: Molecular Biology
Topic: Relationship between structure and function of glucoes
Replies: 12
Views: 767

Re: distinguishing circular and linear DNA

Disregard this, the method I was going to suggest required running on an agarose gel, and the OP mentioned that he was unsure whether a 100kb size genome would run properly. Support you. @Cmgross: before going for agarose gel elctrophoresis or polyacrylamide gel electrophoresis, go for PCR amlifica...

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by greatmicrobiologist
Mon Sep 12, 2011 12:22 pm
 
Forum: Molecular Biology
Topic: distinguishing circular and linear DNA
Replies: 8
Views: 2028

Western Blotting of small molecular weight

... any tips regarding doing Westerns of low-molecular weight proteins. I've been using a 15% gel for SDS-PAGE and I am considering going to a 4-20% linear gradient gel. Will that help? Does anyone think I am having a sample preparation problem? I would appreciate anyones input. Thanks.

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by Homie7
Thu Sep 08, 2011 11:46 am
 
Forum: Molecular Biology
Topic: Western Blotting of small molecular weight
Replies: 4
Views: 1455

Re: genomic rescue construct from BAC DNA

... they suggest 2x tango but the BamHI is reduced to 50-100% activity. What company are the enzymes from? One thing you can do is treat one of your linear fragments with Calf Intestinal Alkalyn Phosphatase (CIP). This will stop re-ligation of your fragment but don't do both fragments (and deactivate ...

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by antonyviridae
Wed Sep 07, 2011 8:33 pm
 
Forum: Molecular Biology
Topic: genomic rescue construct from BAC DNA
Replies: 1
Views: 342
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