Linear

... weight markers in lane one of the gel that I attached. When I do this and graph the Rf values against the molecular weight my graph is not linear. I marked in red where I am measuring from. Can someone please tell me what I am doing wrong?

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... of the experiment (Nt). Because of my experimental setup, I can not count cells in-between. If I could, I would determine a growth rate by doing a linear regression of a log-plot of my cell-nr over time and the slope is then my growth rate. However, with two points only, a regression is senseless. ...

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... the issue (and difference between the two) are not so obvious for us. Our vector is a circular plasmid. Can you elaborate on what you mean by two linear pieces that are homologous to each other? Do you mean using a linear DNA to KO the gene of interest? If so, we had actually successfully applied ...

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Are you sure that the bacteria is capable of homology recombination? Is your vector linearized? What about usage of two linear pieces which are homologous to each other?

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... rise/run, the dy/dx. You can count squares on the graph paper. On the other hand, you can put the values of x and y into a spreadsheet and run a linear regression. If you do that, be sure to go back to the raw data on a graph and make sure the slope makes sense given the graph you see. By the ...

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