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Results from our forumProtein structure... primary,secondary, Tertiary and Quaternary so far this is what i understand by the following terms ok so far i know the primary structure is linear arrange of the amino acids in a peptide chain to show the sequence and number of amino acids in the chain The secondary structure is the arrangement ...
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Re: Relationship between structure and function of glucoes... have different mechanical properties and adopt different confirmations, with poly-α-D-glucose forming curving fibers and poly-β-D-glucose forming linear fibers (as you wrote, Adz, due to different hydrogen-bonding geometries). I don't think there is a similar logic to the L- vs. D- amino acids. ...
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Re: distinguishing circular and linear DNA Disregard this, the method I was going to suggest required running on an agarose gel, and the OP mentioned that he was unsure whether a 100kb size genome would run properly. Support you. @Cmgross: before going for agarose gel elctrophoresis or polyacrylamide gel electrophoresis, go for PCR amlifica...
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Western Blotting of small molecular weight... any tips regarding doing Westerns of low-molecular weight proteins. I've been using a 15% gel for SDS-PAGE and I am considering going to a 4-20% linear gradient gel. Will that help? Does anyone think I am having a sample preparation problem? I would appreciate anyones input. Thanks.
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Re: genomic rescue construct from BAC DNA... they suggest 2x tango but the BamHI is reduced to 50-100% activity. What company are the enzymes from? One thing you can do is treat one of your linear fragments with Calf Intestinal Alkalyn Phosphatase (CIP). This will stop re-ligation of your fragment but don't do both fragments (and deactivate ...
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