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Light microscopy


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Re: Difference between ICC and IF

... made cells permeable). The fluorochrome is detected by fluorescence microscopy, always involving excitation of fluoresence with a defined wavelength band of light and detection of the longer-wave emission from the fluorochrome. Generally for epifluorescence ...

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by jonmoulton
Tue Sep 16, 2008 3:37 pm
 
Forum: Molecular Biology
Topic: Difference between ICC and IF
Replies: 2
Views: 760

Re: UV Microscopy

... these lamps have openings to allow for cooling and a lot of UV escapes thru the openings in the housing. Many materials are fluorescent in visible light as well as UV but it is harder to see with visible light excitation. The light used for excitation is higher energy (shorter wave length) than ...

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by Fauna
Mon Jul 28, 2008 9:24 pm
 
Forum: General Discussion
Topic: UV Microscopy
Replies: 7
Views: 918

Re: microscope magnification

... many ways of which one can observe some specific organelles utilizing a light microscope. Phase contast is one and DIC another and of course there ... and technical forums. This site is a goldmine of information concerning microscopy and extreme close in photography. If you have not a microscope ...

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by Ken Ramos
Sat Jun 28, 2008 5:49 pm
 
Forum: Microbiology
Topic: microscope magnification
Replies: 10
Views: 7274

Important test?

... called a microcapsule because it can be visualized only by electron microscopy unlike the true capsules of some bacteria which are readily visualized by light microscopy. S. aureus strains isolated from infections express high levels of the polysaccharide ...

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by MichaelXY
Wed May 07, 2008 11:48 pm
 
Forum: Microbiology
Topic: Important test?
Replies: 1
Views: 1097

Re: human body cell

... could try to couple the fluorescent dyes with confocal laser scanning microscopy, that allows us to see the positive signal by microscope. This ... many cells that are unique to a certain part of the body, and simple light microscope analysis can allow an experienced researcher to get rough ...

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by biohazard
Sun Jan 06, 2008 6:06 pm
 
Forum: Cell Biology
Topic: human body cell
Replies: 5
Views: 1651
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