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Ligation

Ligation

(Science: dentistry) a process where an archwire is attached to the brackets on your teeth. (surgery) tying a duct or blood vessel with a ligature (as to prevent bleeding during surgery).The joining together of nucleic acids using dna ligase.


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Re: Cell and Molecular Revision Question Help!!!

I don't know, that's question presented to me. As for the Ligation theory, please elaborate? I still don't get it...

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by Didact1
Mon Sep 30, 2013 7:56 pm
 
Forum: Molecular Biology
Topic: Cell and Molecular Revision Question Help!!!
Replies: 4
Views: 2666

Cell and Molecular Revision Question Help!!!

What the "A diploid somatic human cell contains 5 picograms/pg of DNA"? 5 pg of what per pg of DNA? ligation - just calculate the molecular weight of each fragment and then put them into 3:1 ration (by moles)

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by JackBean
Mon Sep 30, 2013 12:08 pm
 
Forum: Molecular Biology
Topic: Cell and Molecular Revision Question Help!!!
Replies: 4
Views: 2666

Cell and Molecular Revision Question Help!!! (2)

... in humans and E.coli, how many more proteins could theoretically be expressed by the haploid human genome than by the haploid E.coli genome? LIGATION THEORY A 100bp fragment is to be cloned into the EcoRI site of pBR328 (4.9 Kb) in order to detect successful insertions using blue/white selection. ...

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by Didact1
Mon Sep 30, 2013 11:35 am
 
Forum: Cell Biology
Topic: Cell and Molecular Revision Question Help!!! (2)
Replies: 0
Views: 1922

Cell and Molecular Revision Question Help!!!

... in humans and E.coli, how many more proteins could theoretically be expressed by the haploid human genome than by the haploid E.coli genome? LIGATION THEORY A 100bp fragment is to be cloned into the EcoRI site of pBR328 (4.9 Kb) in order to detect successful insertions using blue/white selection. ...

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by Didact1
Mon Sep 30, 2013 11:34 am
 
Forum: Molecular Biology
Topic: Cell and Molecular Revision Question Help!!!
Replies: 4
Views: 2666

MOLECULAR CLONING

... ligase removes 3'-phosphates and replaces it with -OH. Thus, synthesized oligos without polynucleotide kinase treatment will participate in the ligation reaction with both their ends having -OH groups. The 3'-OH end of the oligo will form covalent bond with the 5'-phosphate end of the restriction ...

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by kk
Fri Jul 19, 2013 12:50 pm
 
Forum: Molecular Biology
Topic: MOLECULAR CLONING
Replies: 9
Views: 5368
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