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Dictionary » L » Ligase amplification reaction

Ligase amplification reaction

Ligase amplification reaction

(Science: molecular biology) method for detecting small quantities of a target dna, with utility similar to pcr. It relies on dna ligase to join adjacent synthetic oligonucleotides after they have bound the target dna. Their small size means that they are destabilised by single base mismatches and so form a sensitive test for the presence of mutations in the target sequence.


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problem with cloning using pTYB2

... C- termini of the gene and incorporated by PCR amplification. The PCR product and the vector were ... were used to perform ligation with T4 DNA ligase. Since the expected product is not very different ... end) vector:insert, I have tried 1:5 and 1:10, reaction volume is 30 uL. (5).Transformation ...

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by ripplemm
Mon May 05, 2008 8:57 pm
 
Forum: Molecular Biology
Topic: problem with cloning using pTYB2
Replies: 3
Views: 1303


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