Ligase

... to insert double stranded cDNA into this vector? If yes, what are the processes that need to happen in order to make this occur? And can I use DNA ligase to seal the fragments of cDNA and retroviral vector?

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... to do with the splicing nature of the gene.... he suggested that we stop trying to clone the whole gene and look at the splicing nature of ligase 6 before we re-attempt any cloning or over-expression. In order to investigate the splicing nature of the gene, I created primers for each exon ...

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... intron-free clone is the most important part. Moving on from that, I am trying to write some content about the alternative splicing nature of the ligase 6 gene from a gel image that I have taken. If you have the time, I would really appreaciate it if you could help me out! I am finding it all ...

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I have been trying very very hard to clone the ligase 6 gene in Arabidopsis overexpress it. However, it has been proving very difficult due to the high amount of splicing intermediates. I have so far used varying primers on the cDNA of arabidopsis ...

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... fit way where the enzyme is specific to it's substrate and involves the enzyme and the substrate working at an active site? For example DNA Ligase forms phosphodiester bonds between Okazaki fragments... What about when one job involves two enzymes ie. DNA Gyrase and DNA Helicase in unwinding ...

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