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Lids

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How to maintain sterility while preparing petri dishes

... you sterile agar plates in most cases even if done without autoclaves and hoods. Plating the bacteria shouldn't be a problem: even if you open the lids for as long as several minutes, you are likely to get no contaminating bacteria from the air (our 100+ students do so dozens of times in our labs ...

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by biohazard
Mon Jun 24, 2013 8:56 am
 
Forum: Microbiology
Topic: How to maintain sterility while preparing petri dishes
Replies: 8
Views: 4309

How to maintain sterility while preparing petri dishes

... For some of our courses we prepare like 1000 agar plates this way and in most cases none of them have signs of contamination. When the lids are closed and the plates are packed into a plastic bag they also stay sterile for several weeks in the refrigerator. Sterile plastic loops (or ...

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by biohazard
Mon Jun 24, 2013 7:12 am
 
Forum: Microbiology
Topic: How to maintain sterility while preparing petri dishes
Replies: 8
Views: 4309

How to maintain sterility while preparing petri dishes

... bacteria in them, how will I do that sterilely also? I have inoculating loops that I'll sterilize before using but that also requires opening the lids.

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by animus
Thu Jun 13, 2013 10:42 pm
 
Forum: Microbiology
Topic: How to maintain sterility while preparing petri dishes
Replies: 8
Views: 4309

Re: Extraocular Muscles

... then let me know. I have studied your eye diagram as well. Maybe it is like wearing contact lens for the eyes, you know how you can flip your eye lids ha ha ha lol. I don't know but give me a chat back when you get the time Later.

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by CXCR4
Sat Apr 18, 2009 4:21 pm
 
Forum: Human Biology
Topic: Extraocular Muscles
Replies: 2
Views: 5256

Serious Ligation issues :( (Part science part rant)

... 2) Plasmid +insert 3) Insert 4) Cells only. The plates on both occassions are clear as the time they were poured except for condensation on the lids. :oops: :( At this point i'm not sure if its my technique or the prod i'm using. My PCR worked wonderfully and I got nice clean bands for my vector ...

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by dinofernando
Fri Aug 31, 2007 2:46 am
 
Forum: Molecular Biology
Topic: analyzing a ligation before transformation
Replies: 8
Views: 11510
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