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Leishmania

Leishmania

a genus of parasitic flagellated protozoans which causes diseases in animals including humans, most notably leishmaniasis. A type of trypanosome.


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Book recommendation for exam

... 3. Protozoa : Locomotion, nutrition, reproduction; evolution of sex; General features and life history of Paramaecium, Monocystis, Plasmodium, and Leishmania. 4. Porifera : Skeleton, canal system and reproduction. 5. Coelenterata : Polymorphism, defensive structures and their mechanism; coral reefs ...

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by bertstare
Sun Oct 21, 2012 2:19 pm
 
Forum: Zoology Discussion
Topic: Book recommendation for exam
Replies: 3
Views: 4882

designning primers

... gene expression of a specific gene. You would need to perform alignment only when you would have had to distinguish between chitinase gene from leishmania and from other organisms. You may design specific primers directly on the sequences which you extracted from NCBI. For designing highly specific ...

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by wilsontrace
Mon Mar 07, 2011 5:53 am
 
Forum: Molecular Biology
Topic: designning primers
Replies: 5
Views: 2098

designning primers

question is, whether you need to distinguish between chitinase from leishmania and from other organisms. If so, focus on the areas, which differ, if not, you can take any part of the gene.

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by JackBean
Thu Dec 02, 2010 6:35 pm
 
Forum: Molecular Biology
Topic: designning primers
Replies: 5
Views: 2098

designning primers

Find areas that are conserved in leishmania not in the other. Depending on how much similarity there is you can either use that to put your primers (use primer3 available on NCBI website) or only the probe.

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by canalon
Thu Dec 02, 2010 5:21 pm
 
Forum: Molecular Biology
Topic: designning primers
Replies: 5
Views: 2098

designning primers

Hello everyone, I am an undergraduate student. I'm doing project on leishmaniasis( chitinase gene expression on leishmania). Before I start the lab, i need to design my primers for RT-PCR. I have already started using the EBI nad NCBI sites. But I have got ...

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by jajo
Thu Dec 02, 2010 3:04 pm
 
Forum: Molecular Biology
Topic: designning primers
Replies: 5
Views: 2098
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