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Lectin

Lectin

(Science: plant biology) proteins obtained particularly from the seeds of leguminous plants, but also from many other plant and animal sources, that have binding sites for specific mono or oligosaccharides in cell walls or membranes. They thereby change the physiology of the membrane to cause agglutination, mitosis, or other biochemical changes in the cell.

Named originally for the ability of some to selectively agglutinate human red blood cells of particular blood groups. Lectins such as concanavalin a and wheat germ agglutinin are widely used as analytical and preparative agents in the study of glycoproteins.


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Re: Binding proteins to cytoplasmic surface of cellular membrane

Look in the lab book. There's a table on lectins on page 55 that says that Lectin Pisium Sativum binds specifically to Sialic Acid. SO! The question is, does sialic acid only present itself on the outside of the cell membrane, or both inside and out? Also... ...

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by brantozeke
Sat Nov 27, 2010 2:56 pm
 
Forum: Cell Biology
Topic: Binding proteins to cytoplasmic surface of cellular membrane
Replies: 5
Views: 4576

Binding proteins to cytoplasmic surface of cellular membrane

The question is whether the agglutination wil be specific to one type of vesicle or the other. I.e. are there lectin binding site on the cytoplasm site of your membrane.

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by canalon
Mon Nov 22, 2010 11:00 pm
 
Forum: Cell Biology
Topic: Binding proteins to cytoplasmic surface of cellular membrane
Replies: 5
Views: 4576

Binding proteins to cytoplasmic surface of cellular membrane

... vesicles, its suggested that you pass the preparation of mixed vesicles over an affinity column. There is an affinity column made of the lectin pisum sativum from the common pea attached to solid beads for support. Will using this approach to effectively remove the normal vesicles from ...

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by havery01
Mon Nov 22, 2010 9:00 pm
 
Forum: Cell Biology
Topic: Binding proteins to cytoplasmic surface of cellular membrane
Replies: 5
Views: 4576

The Immune System - Recognition in innate immunity. How?

... complex mechanisms of recombination and mutation. The components of the innate immunity (e.g. Toll-like receptor on macrophages, mannose-binding lectin in the plasma) have their specificity encoded on a genomic level, are expressed in similar form on the surface of every cell of a given type, ...

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by biohazard
Tue May 12, 2009 7:43 am
 
Forum: Cell Biology
Topic: The Immune System - Recognition in innate immunity. How?
Replies: 2
Views: 10119

What does hemagglutination look like?

Hi. I'm a PhD student and I've been doing hemagglutination experiments to detect lectin activity in my samples (solubalised cells froma toxic species). My samples lyse live blood cells so I've been using fixed cells (I've fixed them myself). But the fixed cells ...

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by Lornadoon
Thu May 29, 2008 3:59 pm
 
Forum: Cell Biology
Topic: What does hemagglutination look like?
Replies: 2
Views: 4636
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