Lcm virus

depends on the virus....

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How long do a virus live? :roll: :roll: :roll: :roll: :roll: :roll: :roll: :roll:

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... the shRNA sequences. The problem is the results don't seem to traslate well into in vitro systems. I analysed 8 sequences with the assay and made virus with them. The virus / plasmid has an antibiotic resistance gene which is expressed under the same promoter control ( mir30 motif). shRNAs were ...

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pfu=plaque forming units..those are active virus progeny.. you can calculate recombination frequency from such data..

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Is it a function of the Virus used in transfection?

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