Laser

the four nucleotides are tagged with florochromes of different colors(adenine is red, citosine is red etc). The laser can read the colors Contradiction: cytosine and adenine are both red and yet their are of different colors???? Wait... if the laser can read the colors, then it ...

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the four nucleotides are tagged with florochromes of different colors(adenine is red, citosine is red etc). The laser can read the colors

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thanks MrMistery! :D Just one question: then how does the laser and detector in the current dideoxy chain-termination method for sequencing DNA detect whether the dideoxy nucleotide is actually adenine, thymine, cytosine, or guanine if the laser can't ...

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... by size by electrophoresis. The electrophoresis is sensible enough to separate molecule that differ in only one base pair. However, what the laser detector sees at each step is not the individual tagged dideoxynucleotide, but actually the whole DNA molecule. In other words, you only see the ...

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... 1, to the best of my knowledge, the current mehtod of dideoxy chain-termination method for sequencing DNA does use a fluorescence detector (with a laser) to "sense the color of each fluorescent tag as the strands come through. Strands differing by as little as one nucleotide in length can ...

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