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Lane

Lane

a passageway between fences or hedges which is not traveled as a highroad; an alley between buildings; a narrow way among trees, rcks, and other natural obstructions; hence, in a general sense, a narrow passageway; as, a lane between lines of men, or through a field of ice. It is become a turn-again lane unto them which they can not go through. (Tyndale)

Origin: oe. Lane, lone, as. Lone, lone; akin to D. Laan, OFries. Lana, lona.


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Gel electrophoresis

Hey guys! Would anyone know, in each of the Lane 2's, why there is a very large bright fragment that doesn't really confine to the shape of a rectangle? Just a general description would be helpful. Thank you!

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by downingrock
Wed Mar 12, 2014 12:44 pm
 
Forum: Genetics
Topic: Gel electrophoresis
Replies: 3
Views: 1776

Weird - shaped band on western blotting

... to see the cell cycle regulation protein. Recently I got a weird-shaped band on my western blot. It is like an upside-down of smiling face in each lane. I use the same loading buffer, electrophoresis buffer, lysis buffer, and also the same 1st and 2nd antibody for this experiment. I also try to ...

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by namakulaila
Thu Oct 17, 2013 6:17 am
 
Forum: Molecular Biology
Topic: Weird - shaped band on western blotting
Replies: 2
Views: 1481

SDS-PAGE gel and Rf values

Hello, I have to calculate the Rf for the molecular weight markers in lane one of the gel that I attached. When I do this and graph the Rf values against the molecular weight my graph is not linear. I marked in red where I am measuring from. Can someone please ...

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by cschauss
Sun Mar 03, 2013 7:37 am
 
Forum: Cell Biology
Topic: SDS-PAGE gel and Rf values
Replies: 2
Views: 2794

Re:

... image that I have taken. If you have the time, I would really appreaciate it if you could help me out! I am finding it all very difficult; Each lane in the gel has cDNA that has been amplified with a set of primers that cut out an exon from the ligase 6 gene - so each lane should have one exon ...

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by polkadotshorts
Sat Dec 08, 2012 3:52 pm
 
Forum: Genetics
Topic: How to clone a gene with lots of splicing intermediates?
Replies: 6
Views: 3390

Chromatin experiment

Hey, Can someone please confirm my answers before I submit it....correct me if i am wrong instead of beating around the bush! :D explantion 1= lane 2 2= wrong interpretation 3= lane 3 4= lane 1 5= lane 6 6= wrong interpretation 7= lane 3 Which of the following protocols modifications could remove ...

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by 2468
Thu Nov 08, 2012 7:48 pm
 
Forum: Molecular Biology
Topic: Chromatin experiment
Replies: 10
Views: 3322
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