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Lambda phage

Lambda phage --> lambda bacteriophage

(Science: virology) bacterial dna virus, first isolated from E. Coli. Its structure is similar to that of the t even phages.

lambda genetic material consists of a double-stranded dna molecule with 5' twelve-base-pair sticky ends, known as cos sites, which permit circularisation of the dna molecule. It shows a lytic cycle and a lysogenic cycle and studies on the control of these alternative cycles have been very important for our understanding of the regulation of gene transcription.

It is used as a cloning vector, accommodating fragments of dna up to 15 kilobase pairs long. For larger pieces, the cosmid vector was constructed from its ends.


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The use of Cosmids

... But what I don't understand is what role the cos sequence (of the lambda phage) has to do with that? How come introducing the cos sequence in a plasmid makes it able ...

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by Lucanus cervus
Mon Jan 10, 2011 10:07 pm
 
Forum: Genetics
Topic: The use of Cosmids
Replies: 2
Views: 3804

Re: Identify the nucleic acid!

... question may not be answerable with the information given. I see that lambda exonuclease cannot initiate digestion from a nick or a gap (although ... to authentic linear DNA. And it’s been awhile since I’ve thought about phage DNA of any kind, so I’m not sure if the packaged form of P22 DNA is ...

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by blcr11
Wed Feb 20, 2008 9:11 pm
 
Forum: Molecular Biology
Topic: Identify the nucleic acid!
Replies: 5
Views: 4280

Re: Identify the nucleic acid!

... choices because they are all ds DNAs: pBR322 is a standard plasmid; Phage P22 DNA is ds; M13 RF DNA (RF stands for “replicative” form, though ... a ds circle, although the infective DNA is ss and would be resistant to lambda exonuclease. rRNA should be resistant to the exonuclease, does not ...

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by blcr11
Sun Feb 17, 2008 12:16 pm
 
Forum: Molecular Biology
Topic: Identify the nucleic acid!
Replies: 5
Views: 4280

Purifying DNA

... DNA. (Actually, it is possible to separate the individual strands of phage lambda on a CsCl gradient, but that’s a unique case; in general you won’t be able to separate ...

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by blcr11
Sun Oct 14, 2007 7:16 am
 
Forum: Molecular Biology
Topic: Purifying DNA
Replies: 8
Views: 4562

Phage

So far I think it's safe to say that the Cro/Cl system is in the lambda phage families.

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by david23
Tue Aug 28, 2007 11:44 pm
 
Forum: Microbiology
Topic: Phage
Replies: 3
Views: 2631
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