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Lambda bacteriophage

Lambda bacteriophage

(Science: virology) bacterial dna virus, first isolated from E. Coli. Its structure is similar to that of the t even phages.

lambda genetic material consists of a double-stranded dna molecule with 5' twelve-base-pair sticky ends, known as cos sites, which permit circularisation of the dna molecule. It shows a lytic cycle and a lysogenic cycle and studies on the control of these alternative cycles have been very important for our understanding of the regulation of gene transcription.

It is used as a cloning vector, accommodating fragments of dna up to 15 kilobase pairs long. For larger pieces, the cosmid vector was constructed from its ends.


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Need help in Micro!

... Discuss the importance of viral "Host range" 18. Define "bacteriophage" and discuss their importance in destruction of microbes. ... bacteriophage. 22. Describe the lysogenic cycle of the bacteriophage lambda. 23. Compare and contrast the multiplication cycles of DNA & RNA ...

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by whitecaddy26
Thu Feb 09, 2012 7:36 pm
 
Forum: Microbiology
Topic: Need help in Micro!
Replies: 11
Views: 4110

Cloning bacteriphage lambda fragments

We digested bacteriphage lambda with HindIII and EcoRI, ligated the fragments into the vector pHSG398, induced ... recombinant plasmid with HindIII and EcoRI, and determined with fragments of the bacteriophage lambda DNA were cloned. I am using GelAnalyzer to measure the band sizes. ...

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by francisp
Tue Oct 25, 2011 12:26 am
 
Forum: Molecular Biology
Topic: Cloning bacteriphage lambda fragments
Replies: 0
Views: 933

Radioactivity Q

... dNTPs to the 3’ end of DNA, the following experiment was set up: Linear-bacteriophage lambda DNA (48.5kb) at a concentration of 55ug ml^-1 was cut with the restriction enzyme Alu ...

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by monkey07
Tue May 05, 2009 4:18 pm
 
Forum: Molecular Biology
Topic: Radioactivity Q
Replies: 0
Views: 2591

Identify the nucleic acid!

This question is unfair. 1) resistant to digestion with lambda exonuclease: lambda exonuclease has 5' to 3' activity on double stranded ... II M13 DNA - because they are relatively small (4361 bp and 6407 bp) while Bacteriophage P22 DNA is 41661 bp and likely to precipitate. rRNA will precipitate ...

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by Cat
Wed Feb 20, 2008 6:40 pm
 
Forum: Molecular Biology
Topic: Identify the nucleic acid!
Replies: 5
Views: 4280

Identify the nucleic acid!

A nucleic acid sample is resistant to digestion with lambda exonuclease.When heated it does not show typical melting curve of a linear ... at the bottom of the centrifuge tube.The nucleic acid is: a/ccc pBR322 b/Bacteriophage P22 DNA c/rRNA d/RF II M13 DNA

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by vaibhavsmarty
Sat Feb 16, 2008 5:15 am
 
Forum: Molecular Biology
Topic: Identify the nucleic acid!
Replies: 5
Views: 4280
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