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Ladder splint

a flexible splint consisting of two stout parallel wires with finer cross wires.

Synonym: cramer wire splint.

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separation problem with GelRed

... to improve band separation: 1. Reduce the amount of loaded DNA. Overloading of DNA often results in band smearing. The recommended amount for ladders and samples of known concentration is 50-200 ng/lane. If the concentration is unknown, try loading one half or one third of the usual amount ...

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by Biotium
Wed Nov 26, 2014 4:43 pm
Forum: Molecular Biology
Topic: separation problem with GelRed
Replies: 12
Views: 19576

DNA Gel Electrophoresis Issues - Bad Ladder Separation

... in my low molecular weight ladder. I'm following the same protocol used by former members of my lab, and I've seen pictures of their gels and the ladders look perfect. The 2 or 3 largest bands in my ladder are distinct, but all the small bands are fuzzy and indistinguishable. Everyone running ...

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by Etryn
Sat Aug 13, 2011 1:00 am
Forum: Genetics
Topic: DNA Gel Electrophoresis Issues - Bad Ladder Separation
Replies: 4
Views: 9904

separation problem with GelRed

... (We always prestain gels - poststaining is no option because its too inconvenient and timeconsuming - using 1xTAE buffer). Our DNA samples and the ladders aren't separated as sharply as it was when we used ethidium bromide. I already have some ideas what to try to improve the separation (using ...

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by apolll
Sun Aug 23, 2009 8:23 pm
Forum: Molecular Biology
Topic: separation problem with GelRed
Replies: 12
Views: 19576


... you will need to run a similar gel/northern blot. My advice is to load both DNA and RNA weight markers on that RNA gel, and then compare the ladders to each other. This will tell you how you should interpret the ladder from the first gel.

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by snowcapk
Sun Jul 27, 2008 9:40 pm
Forum: Molecular Biology
Replies: 2
Views: 1869

The Fiber Disease

... on a string" images were obtained that were similar to those obtained with nucleosomes reconstituted with four standard histones. DNA ladders with repeats of approximately 10 bp were produced by DNase I digestion, indicating that the DNA was wrapped round the protein complex. Mononucleosomes ...

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by Nadas Moksha
Wed Dec 20, 2006 4:10 pm
Forum: Human Biology
Topic: The Fiber Disease
Replies: 7403
Views: 6245152
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