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Ladder

Ladder

1. A frame usually portable, of wood, metal, or rope, for ascent and descent, consisting of two side pieces to which are fastened cross strips or rounds forming steps. Some the engines play, And some, more bold, mount ladders to the fire. (Dryden)

2. That which resembles a ladder in form or use; hence, that by means of which one attains to eminence. Lowliness is young ambition's ladder. (Shak) fish ladder. See fish. Ladder beetle, a spiral marine shell of the genus scalaria. See scalaria.

Origin: oe. Laddre, as. Hlder, hldder; akin to OFries. Hladder, OHG.leitara, g. Leiter, and from the root of E. Lean, v. See lean, and cf. Climax.


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Campylobacter RFLP troubleshooting

... target with DdeI enzyme (Fermentas) for 8 hours according to the procedure suggested by Fermentas. I am using % 4 Nusieve GTG agarose and 100 bp ladder between 100-1000 bp during electrophoresis and rUn the digests at 90 V for 90 minutes. After staining with etidium broimde, the bands in the ...

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by gislab03
Sat May 04, 2013 1:59 pm
 
Forum: Molecular Biology
Topic: Campylobacter RFLP troubleshooting
Replies: 4
Views: 1908

Protein ladder width increases down the gel?

But the buffer in the new ladder should be the same as the others. The ladder is basically combining 2ul of each of the 9 dye-conjugated proteins (~18ul total). The dye-conjugated protein that I loaded separately and combined in the ladder ...

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by albert23
Wed Feb 27, 2013 5:12 pm
 
Forum: General Discussion
Topic: Protein ladder width increases down the gel?
Replies: 2
Views: 1688

Protein ladder width increases down the gel?

Hi everyone! I'm trying to construct a protein ladder, which are markers used to indicate the molecular weight of proteins when running a SDS-PAGE. I have conjugated several different molecular weight proteins with dyes and ran them on a 12% gel. ...

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by albert23
Wed Feb 27, 2013 12:55 am
 
Forum: General Discussion
Topic: Protein ladder width increases down the gel?
Replies: 2
Views: 1688

DNA

... every histone; then you would see some double-length fragments, triple-length fragments etc. If you run the fragments out on a gel you would see a ladder and analysis of the masses of the ladder rungs would reveal that they are integer multiples of the mass of the smallest rung -- that is, the ...

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by jonmoulton
Thu Feb 14, 2013 4:32 pm
 
Forum: Genetics
Topic: DNA
Replies: 8
Views: 6023

Brightness of the 3 Kb band - Why?

What is the purpose of the brightness of the 3 Kb band in a DNA ladder? I know it will help permit easy identification of the band to use it as a reference point, but what other purposes does the brightness serve? Thanks in advance!

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by Entropy
Sun Nov 25, 2012 11:36 pm
 
Forum: Cell Biology
Topic: Brightness of the 3 Kb band - Why?
Replies: 1
Views: 974
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