Laci

... matter what they look like). B. Agreed with blcr11 C. Which cells will grow in the medium with PG as the only source of carbon and energy? А. lacI −. B. lacZ −. C. lacy −. D. lacZ − lacy −. E. lacI − lacZ −. The answer is A: PG is not an inductor, so it will not be able to inactivate the repressor. ...

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... galactosidepermease which carries out lactose transport to the cell, lacA encodes galactoside-transacetylase. Lac operon is under the control of LacI (repressor), which is inactive in the presence of lactose (inductor). There is a wide diversity of the chemical lactose analogs, for example: Orthonitrophenyl-β-D-galactoside ...

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... reverse complementary sequences, highlight differences... Auto-annotate constructs: this tool predicts all common features (e.g. promoters, ORFs, lacI, Amp, Rep origin ...) and converts your sequences to fully annotated GenBank format. Save your data on-line for further studies!

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... the correct insert according to sequencing, but I cant express the protein. The vector demands special cells for expression because it lacks the lacI repressor. The gene contains alot of rare codons but even with the rosetta cells not a single tiny protein is produced. Does anyone had the same ...

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