L cell

Hi guys, I'm a new guy in molecular biology. I plan to express a scfv in S2 cell using the plasmid pMT/bip/V5-His A (with enzyme site BglII and XbaI). Because there's a Bip signal sequence ahead of the enzyme site BglII, so I CAN NOT add Kozak sequence in the ...

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it is based on the intensity of vu rays as the pigment melanin is purely designed for protection from uv rays is is deffinetey effected by the intensity of the light

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You will get soluble part, precipitated proteins and cell debris, i.e. insoluble polysaccharides and stuff. However, I would first care how to get it into solution.

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Hi there, I have to do a cell wall extraction on bacteria soon and my protein of interest is cell wall anchored. My supervisor told me to do TCA precipitation (haven't done before) but I assume it's too isolate the proteins within the ...

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Just a question about mtDNA: According to the endosymbiontic theory mitochondria were ancient bacteria that survived in an ancient eukaryotic cell and continued to live as part of it as an organelle. So, I suppose, in any eukaryotic cell the mtDNA should be relatively conserved. However, the ...

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