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L-cysteine-cystine c-s lyase

L-cysteine-cystine c-s lyase

(Science: enzyme) C-des - cysteine desulfhydrase; a pyridoxal phosphate- containing enzyme from synechocystis involved information of fe-s cluster of ferredoxin; catalyses the breakdown of l-cysteine to yield sulfide, pyruvate and ammonia; increased activity with cystine, yielding cysteine persulfide as the primary product; mw about 43 kd

registry number: EC 4.4.1.-

Synonym: c-des lyase


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Halophilic bacteria growth

... not enough for the Na+ -dependent transport---> decreased cell division - at high/low [] the water availability causes the cell to crenate/lyase? What else can I add? THnaks

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by Nicolodn
Sat Oct 16, 2010 1:43 am
 
Forum: Cell Biology
Topic: Halophilic bacteria growth
Replies: 0
Views: 1829

Re:

your primers can anneal two genes? Sorry, I didn't exactly express my opinion. I do two control experiments: 1, Amplify isocitrate lyase (using primer pairs A), to test whether the genome templete is OK. 2, Amplify anti-kanamycin gene using PKD46 as templete (using primer pairs B), to test ...

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by MXH
Thu Apr 08, 2010 10:32 am
 
Forum: Microbiology
Topic: Fake positive colony in lamda RED reconbination, why?
Replies: 14
Views: 7368

Fake positive colony in lamda RED reconbination, why?

Sorry, By use these words, "like anti-Kanamycin gene", I mean the size of isocitrate lyase gene is similar to anti-Kanamycin gene

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by MXH
Thu Apr 08, 2010 8:06 am
 
Forum: Microbiology
Topic: Fake positive colony in lamda RED reconbination, why?
Replies: 14
Views: 7368

Fake positive colony in lamda RED reconbination, why?

Thank you, JackBean I can amplify the isocitrate lyase gene (1.3kb, like anti-Kanamycin gene)from all positive control with the same conditions. But I cann't amplify anti-Kanamycin gene from all positive colonies. I was frustrated.

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by MXH
Thu Apr 08, 2010 3:16 am
 
Forum: Microbiology
Topic: Fake positive colony in lamda RED reconbination, why?
Replies: 14
Views: 7368

gel electrophoresis & assays help please

... able to contact him at all so he can explain the table of number that i have in front of me! The practical was about alginate and how the alginate lyase gene can be broken down. we had to take the alginate lyase gene Aly from Klebsiella pneumonia in insert it into cosmid vector to produce pSP1. ...

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by RachBio
Fri Feb 06, 2009 11:32 pm
 
Forum: Molecular Biology
Topic: gel electrophoresis & assays help please
Replies: 6
Views: 7703
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