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Knockout

Knockout

(Science: genetics molecular biology) informal term for the generation of a mutant organism in which the function of a particular gene has been completely eliminated (a null allele).

See: homologous recombination, transposon.


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... embryos in which the Pitx1 gene was mutated, making the Pitx1 protein non-functional from the start of mouse development. The images of the Pitx1 “knockout” mouse shown below on the right reveal striking differences in morphology of the jaw and hind limbs between the normal and Pitx1 -/- experimental ...

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by scienceguy914
Mon Sep 08, 2014 1:44 am
 
Forum: General Discussion
Topic: Help With
Replies: 0
Views: 51

Conditional knockout mouse

Hello, I am using transgenic mouse where my gene of interest is floxed. When I cross this mouse line with Tamoxifen inducible Cre line, CAGGCre-ERTM, I get mice with with heterozygous Cre and floxed gene of interest. The experimental plan was to isolate myoblasts from these mice and induce Cre in ce...

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by aji01
Wed Feb 20, 2013 11:25 am
 
Forum: Genetics
Topic: Conditional knockout mouse
Replies: 1
Views: 1569

Re: Knockout mice

"Does this imply that all the somatic cells in the mice contain 1 copy of the functional gene and 1 copy of the non-functional gene? " Yes. "Would this then mean that each cell is expressing the protein to some extent?" Likely yes, though this can be complicated by the biology, e...

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by jonmoulton
Wed Feb 06, 2013 3:51 pm
 
Forum: Genetics
Topic: Knockout mice
Replies: 5
Views: 4293

Knockout mice

If one parent is a wild-type mouse and the other is a homozygous knockout mouse, their offspring will be heterozygous at the knockout gene. The mouse will likely produce the protein from the wild-type copy of the gene, but depending on how the gene is regulated ...

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by jonmoulton
Tue Feb 05, 2013 5:29 pm
 
Forum: Genetics
Topic: Knockout mice
Replies: 5
Views: 4293

Knockout mice

Can someone please explain how it is possible to have a heterozygous knock out mouse? If a gene is "knocked-out," how can the mouse be heterozygous for the gene? Will the mouse still produce express this "knocked-out" gene? Thanks very much!

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by cassieconte
Tue Feb 05, 2013 4:54 pm
 
Forum: Genetics
Topic: Knockout mice
Replies: 5
Views: 4293
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