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Kinase

Kinase

(Science: enzyme molecular biology) Widely used abbreviation for phosphokinase, an enzyme catalysing transfer of phosphate from atp to a second substrate usually specified in less abbreviated name, for example creatine phosphokinase (creatine kinase), protein kinase. Serine / threonine kinases phosphorylate on serine or threonine residues, tyrosine kinases on tyrosines.


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Cell signalling inhibitors

... for me some cell signalling inhibitors for C. elegans (or generic) and tell me the enzyme or receptor that is inhibited? (Preferably for tyrosine kinase, G-protein, JAK/STAT and Phosphatidylinositol 3-kinase) Thanks :D

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by billyfisher100
Wed Jan 08, 2014 7:35 pm
 
Forum: Cell Biology
Topic: Cell signalling inhibitors
Replies: 1
Views: 570

Re:

... another textbook and you were right! Unlike what I first thought, glycolysis can't take place once glycogenolysis has started because the pyruvate kinase is inhibited by glucagon and adrenaline, so PEP can't turn into pyruvate anymore. Which is only adding to my confusion...

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by Aymeric
Sat Dec 14, 2013 11:51 pm
 
Forum: Human Biology
Topic: When does gluconeogenesis kick in during physical activity?
Replies: 12
Views: 2484

Re:

... which quickly is not the case anymore as soon as physical activity starts, right? And it is also activated by calcium (through the phosphorylase kinase) due to muscle contraction. Or am I mixing everything up?

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by Aymeric
Fri Dec 13, 2013 2:46 pm
 
Forum: Human Biology
Topic: When does gluconeogenesis kick in during physical activity?
Replies: 12
Views: 2484

MOLECULAR CLONING

... have 5'-OH and 3'-phosphate ends... DNA ligase removes 3'-phosphates and replaces it with -OH. Thus, synthesized oligos without polynucleotide kinase treatment will participate in the ligation reaction with both their ends having -OH groups. The 3'-OH end of the oligo will form covalent bond ...

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by kk
Fri Jul 19, 2013 12:50 pm
 
Forum: Molecular Biology
Topic: MOLECULAR CLONING
Replies: 9
Views: 4108

MOLECULAR CLONING

... double stranded oligos need to be modified in any way before the ligation reaction. And yes, they need a 5'-phosphate added, by polynucleotide kinase, so the ligase can actually generate the covalent link.

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by kk
Wed Jul 10, 2013 2:58 pm
 
Forum: Molecular Biology
Topic: MOLECULAR CLONING
Replies: 9
Views: 4108
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