Kcal

... get a Tm of 57oC w/ a G/C content of 28%. Lastly, when I finally got my primers to a Tm of ~58% my delta G self-dimers (homo-dimers) was -9.5 kcal/mol. Isn't the deltaG supposed to be about -6?

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All well except for the begining. Average metabolic waste is 2000 KCal, not 2000 Cal. Sometimes dietists speak of calories meaning Kcal, just for briefing. That's all.

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... molecule. Since sucrose contains two molecules that can enter the cellular respiration process (36 ATP x 2 Molecules = 72 ATP per Sucrose) 2,000 KCal for our diet / 4 KCal in a gram of sucrose = 500g of sucrose. 500g sucrose / 342.3g molecular weight of sucrose = 1.4607 moles of sucrose. 1.4607 ...

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You need 2000 Kcal a day irrespective whether they come from. Our calculations show that the ammount of ATP involved would be very similar on a diet based only on sugars and on a diet based only on fat. Hence diet composition is ...

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So, as noted above: 2,000 KCal for our diet / 4 KCal in a gram of sucrose = 500g of sucrose. 500g sucrose / 342.3g molecular weight of sucrose = 1.4607 moles of sucrose. 1.4607 moles of sucrose x 6.02 x 10^23 = 8.793 x 10^23 molecules ...

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