Isolation

Okay, after doing some reading, I have come up with a different plan for quantifying HIF-1 alpha, which involves running an ELISA after performing a nuclear extraction. However, all the protocols and kits I have seen recommend 1-10 million cells for a good extraction. I am likely going to be using m...

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Hi, I am trying to quantify HIF 1 alpha protein level. Our scaffold is not a polystyrene plate, and the cells are not grown as 2D monolayers (I am looking at cell aggregates to be specific). Our original plan is to isolate the protein using trizol extraction reagent, then run an ELISA on HIF 1 alpha...

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can someone explain on reinforcement of prezygotic isolation related to mating calls in male frogs?

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... and I would like you to help me figure it out. Sometimes we read about solution contents for example the enzyme solution used in protoplast isolation, well in the paper its written like this 20 mM MES 0.4 M mannitol 20 mM KCl 10 mM CaCl2 0.1% BSA 1.5% Cellulase and so on... I know how to ...

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... sequence and ligate them together to generate the full length gene which I would then clone and amplify. However, there were troubles with the isolation of the bands. I ran the experiment a number of times and didn't always get the same result (despite the exact same procedure and minimal scope ...

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