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Campylobacter RFLP troubleshooting

I do not have any purpose of cloning.My aim is to type 300 Campylobacter strains molecularly and look for similarity between isolates; in short my aim is molcular typing. I am using RFLP just for that. Not for cloning or for something genetical method.

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by gislab03
Tue May 07, 2013 4:06 pm
Forum: Molecular Biology
Topic: Campylobacter RFLP troubleshooting
Replies: 4
Views: 1974

Re: Theories - Origin of Life

... an sound empirical observation. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC383146/ The low level of occurrence of tetracycline resistance among isolates from wild animals is presumably due to their low exposure to these antibiotics. Most of these isolates either had a high level of resistance ...

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by scottie
Fri Aug 17, 2012 3:46 pm
Forum: Evolution
Topic: Theories - Origin of Life
Replies: 560
Views: 907255

Vaccine for HIV

... can evade the two major arms of the adaptive immune system; humoral (antibody-mediated) and cellular (mediated by T cells) immunity. Second, HIV isolates are themselves highly variable. HIV can be categorized into multiple clades and subtypes with a high degree of genetic divergence. Therefore, ...

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by suzidoc8
Mon Jul 23, 2012 2:47 pm
Forum: Microbiology
Topic: Vaccine for HIV
Replies: 1
Views: 7620

Hypothetical experiment

... on agar/same media for isolated colonies, etc. Prepare DNA for sequencing. Sequence whole genomes of samples from all 3 experiments. 6. Test all isolates for ability to utilize chemical introduced in c) by growing all isolates (from all experiments) in minimal media with this chemical as the ...

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by Cat
Tue Dec 06, 2011 8:54 pm
Forum: Evolution
Topic: Hypothetical experiment
Replies: 6
Views: 4430

3 models for genetic isolation

... of a population • Allopatric and sympatric speciation- Something happens (i.e. geographical isolation, ecological isolation) to a population that isolates the gene flow. The two subpopulations are separated whereas there will be no reproduction. Allopatric refers to a geographic separation of ...

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by hannahzarzar
Wed Aug 31, 2011 5:31 am
Forum: Evolution
Topic: 3 models for genetic isolation
Replies: 1
Views: 3591
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