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Isolated

Isolated

Placed or standing alone; detached; separated from others. Isolated point of a curve.

(Science: geometry) see Acnode.


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amplification in no RT control

I am doing some qRT PCR to assess changes in bacterial gene expression following exposure to antimicrobials. RNA has been isolated by trizol method and subsequently 'cleaned' on column and finally been DNase treated prior to reverse transcription. I am getting amplification in all of ...

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by biomart
Fri Mar 20, 2015 1:58 am
 
Forum: General Discussion
Topic: amplification in no RT control
Replies: 1
Views: 51

Re: 6 fingers

... is intercalary hexadactyly. Hexadactyly in itself can be innocuous, absolutely harmless and very easily remedied, when the hexadactyly is an isolated finding and the baby is otherwise entirely normal. Ulnar hexadactyly with just a rudimentary tag of a sixth digit, for instance, can be very ...

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by BrentElmer
Fri Jan 30, 2015 7:10 am
 
Forum: Genetics
Topic: 6 fingers
Replies: 5
Views: 5907

An Overview of Embryonic Stem Cells

... also be induced to differentiate into derivatives of all three embryonic germ layers; endoderm, ectoderm and mesoderm. Murine ES cells were first isolated in 1981 by the use of culture techniques based on experience with the culture of EC cells . ES cells are derived from the pre-implantation ...

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by 2444256703
Thu Jan 29, 2015 6:48 am
 
Forum: Cell Biology
Topic: An Overview of Embryonic Stem Cells
Replies: 0
Views: 582

Re: holographic origin of life

... chemicals would diffuse around not allowing complex reactions to be sustained. Complex arbitrary reactions can be sustained only in an isolated place (remember the paradigm with the flask), but not for long because of the lack of chemical resources. So my described model can only develop ...

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by minasole
Wed Dec 31, 2014 8:13 pm
 
Forum: Evolution
Topic: holographic origin of life
Replies: 15
Views: 27291

Help: Confused with primers in a PCR of a vector?

... on. We recently ligated a gene into a pBS vector and popped it into some E. coli cells. We looked for positive clones via blue/white screening and isolated/purified the respective plasmid. Then, we carried out a PCR reaction of the pBS-gene (our clone). This PCR reaction is something I do not really ...

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by keetner
Sat Mar 15, 2014 3:21 am
 
Forum: Molecular Biology
Topic: Help: Confused with primers in a PCR of a vector?
Replies: 2
Views: 4618
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