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to separate from other persons, materials or objects.

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RNA isolation from brain sections

I'm looking to isolate RNA from brain sections under two conditions. First are punches from fresh vibratome slices. I've been told that the time it takes to cut the sections and then punch them is too long for good RNA isolation. ...

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by protoemics
Fri Mar 20, 2015 2:19 am
Forum: General Discussion
Topic: RNA isolation from brain sections
Replies: 0
Views: 64

An Overview of Embryonic Stem Cells

... also be induced to differentiate into derivatives of all three embryonic germ layers; endoderm, ectoderm and mesoderm. Murine ES cells were first isolated in 1981 by the use of culture techniques based on experience with the culture of EC cells . ES cells are derived from the pre-implantation ...

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by 2444256703
Thu Jan 29, 2015 6:48 am
Forum: Cell Biology
Topic: An Overview of Embryonic Stem Cells
Replies: 0
Views: 733

Mycobacterium vaccae

... adding dirt to my diet and hoping I get mycobacterium vaccae along with who knows what else. If anybody could help me either by telling me how to isolate this myself or by doing so and allowing me to purchase it I would appreciate it. As far as I know it is harmless, common in soil, and so I am ...

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by Popinjay
Sun Nov 09, 2014 5:03 am
Forum: Microbiology
Topic: Mycobacterium vaccae
Replies: 0
Views: 705

Re: Streptococcus sanguis??

Don;t understand why you wasted so much media and time with irrelevant tests. Please determine if this isolate shows alpha hemolysis on blood agar.

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by Cepacia
Sat Dec 21, 2013 1:22 pm
Forum: Microbiology
Topic: Streptococcus sanguis??
Replies: 1
Views: 3876

Re: Another molecular question

... you can determine the amino acids. When you know these, you can figure out the nucleotide sequence. Next of you can hibridize a probe, and isolate your piece of dna maybe?

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by aapje10
Tue Aug 20, 2013 8:19 pm
Forum: Molecular Biology
Topic: Another molecular question
Replies: 3
Views: 1674
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