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Isoamyl

Isoamyl --> amyl

(Science: chemistry) a hydrocarbon radical, C5H11, of the paraffine series found in amyl alcohol or fusel oil, etc.

Origin: L. Amylum starch _ -yl. Cf. Amidin.


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How does chloroform bind to proteins and lipids?

I am aware that in PCR Chloroform from either Phenol: Chloroform: Isoamyl or Chloroform: Isoamyl binds to protein and lipids causing them to precipitate. Where exactly does the Chloroform bond (mechanism, please)??

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by Melstmu2010
Wed Jun 22, 2011 4:01 pm
 
Forum: General Discussion
Topic: How does chloroform bind to proteins and lipids?
Replies: 2
Views: 1976

Centrifugation

... requires centrifugation during the isolation procedure. Specifically, I centrifuge for 10 minutes at 14,000 rpm after adding phenol: chloroform: isoamyl to sample DNA. My question is, why is a speed of 14,000 rpm necessary? I've been told that it is not necessary in all protocols, but i'm asking ...

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by Melstmu2010
Wed Jun 22, 2011 2:56 pm
 
Forum: General Discussion
Topic: Centrifugation
Replies: 4
Views: 1977

Can any one clarify my question with DNA

... Mg2+ and Ca2+ from DNase enzymes and prevent DNA degradation. TRIS - keeps DNA soluble in water by keeping it deprotonated Phenol:chloroform isoamyl alcohol - to remove proteins

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by Cat
Fri Mar 14, 2008 4:12 pm
 
Forum: Molecular Biology
Topic: Can any one clarify my question with DNA
Replies: 2
Views: 3006

Can any one clarify my question with DNA

... for the extraction of DNA we use the following reagents, could you tell me what are their different roles? NaCl, EDTA, Tris, CTAB, Chloroform/Isoamyl alcohol. Similarly why is the pH adjusted to 8?" Now as a beginning of an answer: NaCl is used to prevent the co-precipitation of DNA and ...

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by canalon
Fri Mar 14, 2008 3:25 pm
 
Forum: Molecular Biology
Topic: Can any one clarify my question with DNA
Replies: 2
Views: 3006

Can any one clarify my question with DNA

... buffer containing NaCl, EDTA, Tris n CTAB whts d role of those chemicals n n why adjusting d buffer pH to 8 n I feel same doubt with chloroform isoamyl alcohol(their role)

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by shivakumar
Fri Mar 14, 2008 6:29 am
 
Forum: Molecular Biology
Topic: Can any one clarify my question with DNA
Replies: 2
Views: 3006
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