Dictionary » I » Ions

Ions

Ions

atoms or radicals having a charge of positive (cation) or negative (anion) electricity owing to the loss (positive) or gain (negative) of one or more electrons.


Please contribute to this project, if you have more information about this term feel free to edit this page



Results from our forum


Questions about DNA dialysis

... except the bit of volume stuck in the gizmo if you have small volumes, and perhaps a tad stuck in the pores. It should work by simply allowing the ions to diffuse across the membrane so that the initial volume becomes the final volume, and the salts are diluted proportionately. Like, V1/V2 * inititial ...

See entire post
by biomart
Wed Mar 11, 2015 7:43 am
 
Forum: Bioinformatics
Topic: Questions about DNA dialysis
Replies: 1
Views: 284

Re: principle of error-prone PCR???

I don't know much about this problem. But all I know is that only metal ions that bind to ECE with high affinity can affect the enzyme. Welcome to discuss with me more.

See entire post
by AmyCollins
Thu Feb 05, 2015 9:23 am
 
Forum: Molecular Biology
Topic: principle of error-prone PCR???
Replies: 5
Views: 2474

Questions about DNA dialysis

Dear all, I would like to use dialysis to exchange ions in my DNA. For this I bought Slide-A-Lyzer mini pots for the dialysis. First, I am looking for more information about the theoretical background of dialysis. Can you give me some literature hints, ...

See entire post
by protoemics
Thu Feb 05, 2015 7:56 am
 
Forum: Bioinformatics
Topic: Questions about DNA dialysis
Replies: 1
Views: 284

principle of error-prone PCR???

Thanks so much for your answer. But how exactly the size of Mn ions affect the Taq enzyme? Did they changes the fourth-level structure of the Taq enzyme, such as combine with the regulatory subunit to control the activity, or something else. I mean, it ...

See entire post
by feixiaorou
Wed Oct 01, 2014 3:51 am
 
Forum: Molecular Biology
Topic: principle of error-prone PCR???
Replies: 5
Views: 2474

principle of error-prone PCR???

... with one metal while with the other it can cleave also other substrates as ATP etc.), the reason probably being the size of the metal ions and other properties.

See entire post
by JackBean
Tue Sep 30, 2014 8:19 am
 
Forum: Molecular Biology
Topic: principle of error-prone PCR???
Replies: 5
Views: 2474
View all matching forum results

This page was last modified 21:16, 3 October 2005. This page has been accessed 15,727 times. 
What links here | Related changes | Permanent link