Ionisation

... in the laboratory, the activity of enzymes is strongly affected by changes in pH and temperature. Changes in pH: alther the state of the ionisation of the charged amino acids; that play a crucial role in the substrate binding and/or the catalytic action itself. As seen experimentally, ...

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... pKa The information which was given and is required to calculate pKa are: 1) Change in absorbance with pH is directly proportional to change in ionisation of o-nitrophenol with pH. 2) pKa of o-nitrophenol is the pH at which the change in ionisation is half complete (NB [base]=[acid]). 3) O-nitrophenol ...

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... So far I applied three experiments, so I got three times radiation. Well... :roll: good for you that gamma radiation has a low capacity for ionisation :) Didn't got above the maximum radiation dosage equivalents ?

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