Ion exchanger

... for the column is 20mM TRIS(pH 8.5)... and I salt out using KCl across the concentration gradient of 0.1M - 1.0M... and the column is a anion exchanger, sepharose (Q). Now the problem is I'm not seeing my protein after elution, roughly about 20 times the volume of the column has been passed ...

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... THE FACTOREY: We present the nucleotide sequence of a 5248 bp-long region of the mitochondrial (mt) genome of the dermatophyte Trichophyton rubrum. ... containing the novel PRONE domain (plant-specific Rop nucleotide exchanger), which is exclusively active towards members of the Rop subfamily. ...

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