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Ion exchange chromatography

Ion exchange chromatography

(Science: procedure) separation of molecules by absorption and desorption from charged polymers. An important technique for protein purification.

For small molecules the support is usually polystyrene, but for macromolecules, cellulose, acrylamide or agarose supports give less non-specific absorption and denaturation. Typical charged residues are CM carboxymethyl) or DEAE (diethylaminoethyl).


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ion exchange chromatography

Hi there, I plan on doing IEC tomorrow and need to dialyze my protein to keep a negative charge...the plan is to dialyze in Tris pH 8 (pi of my protein is 6) does that sound right...I don't have to add NaCl to the buffer right (NaCl is what I'm eluting with)..I seem to have written in my book "...

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by biology_06er
Wed Nov 02, 2011 3:20 am
 
Forum: General Discussion
Topic: ion exchange chromatography
Replies: 1
Views: 457

Re: Ion Exchange Chromatography

Ok...all that makes alot of sense...thank you!!

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by Elinor
Sat Oct 04, 2008 4:14 pm
 
Forum: Molecular Biology
Topic: Ion Exchange Chromatography
Replies: 2
Views: 2569

Re: Ion Exchange Chromatography

I don't know why it should be worse for ion exchange than for any other kind of column chromatography. If you let the fluid level drop below the top boundary, ...

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by blcr11
Sat Oct 04, 2008 12:33 pm
 
Forum: Molecular Biology
Topic: Ion Exchange Chromatography
Replies: 2
Views: 2569

Ion Exchange Chromatography

Hi everyone, I have a question about ion exchange chromatography: When using the ion exchange column in this method, why ...

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by Elinor
Fri Oct 03, 2008 3:03 am
 
Forum: Molecular Biology
Topic: Ion Exchange Chromatography
Replies: 2
Views: 2569

Re: Are these right?--won't take long..

... just written out the ones I chose: affinity chromatography is a purification technique that: separates proteins according to size ... according to their hydrophilicity involves ion-exchange chromatography results in protein samples that ...

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by MrMistery
Sat Nov 03, 2007 3:57 pm
 
Forum: Molecular Biology
Topic: Are these right?--won't take long..
Replies: 3
Views: 3057
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