Ion exchange chromatography

Hi there, I plan on doing IEC tomorrow and need to dialyze my protein to keep a negative charge...the plan is to dialyze in Tris pH 8 (pi of my protein is 6) does that sound right...I don't have to add NaCl to the buffer right (NaCl is what I'm eluting with)..I seem to have written in my book "...

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Ok...all that makes alot of sense...thank you!!

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I don't know why it should be worse for ion exchange than for any other kind of column chromatography. If you let the fluid level drop below the top boundary, ...

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Hi everyone, I have a question about ion exchange chromatography: When using the ion exchange column in this method, why ...

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... just written out the ones I chose: affinity chromatography is a purification technique that: separates proteins according to size ... according to their hydrophilicity involves ion-exchange chromatography results in protein samples that ...

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