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Introns

Introns

Non-coding, intervening sequences of dna that are transcribed, but are removed from within the primary gene transcript and rapidly degraded during maturation of messenger rna. most genes in the nuclei of eukaryotes contain introns, as do mitochondrial and chloroplast genes.


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Re: Does complementary DNA include only coding sequences ?

cDNA can contain 5' and/or 3' UTR sequence but not introns.

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by jonmoulton
Mon Oct 28, 2013 3:46 pm
 
Forum: Genetics
Topic: Does complementary DNA include only coding sequences ?
Replies: 4
Views: 1826

Re:

... DNA. People call junk DNA all DNA with unknown function it means all DNA which does not produce protein or has a regulatory function. For example introns, they are segments of DNA from a gene which are transcribed to RNA, then cut out and disappear (They do not code for protein). Thir funcion ...

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by wiliamjack703
Thu Aug 22, 2013 6:36 am
 
Forum: Genetics
Topic: Junk DNA
Replies: 7
Views: 3483

overexpression xylanase gene

1) this can have several guidelines, since bacteria have no introns, you can easily clone the gene directly from genomic DNA. This clone into any vector (either one for keeping the gene or directly into overexpression one). Transform the vector into E. ...

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by JackBean
Thu Dec 13, 2012 11:43 am
 
Forum: Molecular Biology
Topic: overexpression xylanase gene
Replies: 2
Views: 1591

Re:

... want to express it? In what system? If you wanted to use eukaryotic system (yeast or best plants), you could clone genomic version including the introns and let the cell to solve your problem. Hello, thank you for your response :D sorry, this is my first post on this website, so I am not quite ...

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by polkadotshorts
Sat Dec 08, 2012 3:52 pm
 
Forum: Genetics
Topic: How to clone a gene with lots of splicing intermediates?
Replies: 6
Views: 3189

How to clone a gene with lots of splicing intermediates?

... want to express it? In what system? If you wanted to use eukaryotic system (yeast or best plants), you could clone genomic version including the introns and let the cell to solve your problem.

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by JackBean
Sat Nov 24, 2012 8:07 pm
 
Forum: Genetics
Topic: How to clone a gene with lots of splicing intermediates?
Replies: 6
Views: 3189
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