Introns

1) this can have several guidelines, since bacteria have no introns, you can easily clone the gene directly from genomic DNA. This clone into any vector (either one for keeping the gene or directly into overexpression one). Transform the vector into E. ...

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... want to express it? In what system? If you wanted to use eukaryotic system (yeast or best plants), you could clone genomic version including the introns and let the cell to solve your problem. Hello, thank you for your response :D sorry, this is my first post on this website, so I am not quite ...

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... want to express it? In what system? If you wanted to use eukaryotic system (yeast or best plants), you could clone genomic version including the introns and let the cell to solve your problem.

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... on the longest transcript). In addition, assuming I have only a single transcript, which tables do you suggest to use in order to fetch the exons\introns positions within the transcript\cDNA? Thanks in advance, Noa

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... proteins bind to nucleic acids. The question is, how to discriminate those bound in spliceosome from others. One way could be specific binding to introns (although that could show proteins bound to gDNA), but with that you could have false results because of one-case-only binding/non-binding. ...

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