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How to clone a gene with lots of splicing intermediates?

... very hard to clone the ligase 6 gene in Arabidopsis overexpress it. However, it has been proving very difficult due to the high amount of splicing intermediates. I have so far used varying primers on the cDNA of arabidopsis in order to get some fragments that I could then ligate together. I have ...

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by polkadotshorts
Sat Nov 24, 2012 6:49 pm
Forum: Genetics
Topic: How to clone a gene with lots of splicing intermediates?
Replies: 6
Views: 4446

Re: Gluconeogenesis

... to yield the same product. Some small amount of glucose may be found in your cells with no substrate from gluconeogenesis of residual metabolic intermediates? Acetyl-CoA, Oxaloacetate, malate, PEP or isomerization from fructose?

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by bytesyzechic
Wed Nov 14, 2012 11:07 pm
Forum: Cell Biology
Topic: Gluconeogenesis
Replies: 8
Views: 9375

Re: Help - Why can't DNA be parallel?

... Also, I am horrible with chemistry related type things...so how would mesomeric effects play a part in this? Assuming you're talking about intermediates that could occur...? I'm a bit lost on here.

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by keetner
Tue Oct 02, 2012 9:07 pm
Forum: Molecular Biology
Topic: Help - Why can't DNA be parallel?
Replies: 7
Views: 7355

Reasons for Skepticism on the Endosymbiosis?

... back into the mitochondria in order to be functional. All in all, mechanistic scenarios for the endosymbiotic theory imply many non-functional intermediates or would just be plain harmful to an organism. Therefore, the endosymbiotic theory is in contrast with the concept of gradualism that ...

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by SuperfluousAvatar
Thu Jan 12, 2012 7:43 pm
Forum: Cell Biology
Topic: Reasons for Skepticism on the Endosymbiosis?
Replies: 0
Views: 7606

Re: Camouflage in nature

... "fully formed". Not all of these intermediate bugs will survive to reproduce. But STATISTICALLY in the population of a million bugs, the intermediates are more likely to survive. In time, their progeny will be better intermediate forms, and their progeny will be even better, until we ...

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by Gavin
Fri Nov 11, 2011 6:57 am
Forum: Evolution
Topic: Camouflage in nature
Replies: 16
Views: 16631
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