Dictionary » I » Intercalate

Intercalate

Intercalate

1. To insert, as a day or other portion of time, in a calendar.

2. To insert among others, as a verse in a stanza; specif.

(Science: geology) to introduce as a bed or stratum, between the layers of a regular series of rocks. Beds of fresh water shells . . . Are intercalated and interstratified with the shale. (Mantell)

Origin: L. Intercalatus, p. P. Of intercalare to intercalate to intercalate; inter between _ calare to call, proclaim. See Calendar.


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EtBr

I'm sure EtBr intercalate with secondary structures of RNA, so that wouldn't work for my purpose... How about the use of restriction enzymes? Do you guys know the enzyme which would cut almost every kind of DNA leavind RNA intact?

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by HELISA
Thu Sep 13, 2007 2:18 pm
 
Forum: Molecular Biology
Topic: How to differentiate between RNA and DNA
Replies: 12
Views: 9252

How many SYBR-Green molecules intercalate in DNA?

If I have a DNA with a known number of base pair is it possible to estimate how many SYBR-Green molecules can intercalate in it? I don't know much about chemistry, but probably it can be determined from the structure of the dye molecule? By the way, does anyone know where to find ...

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by silin
Fri Sep 01, 2006 5:57 am
 
Forum: Molecular Biology
Topic: How many SYBR-Green molecules intercalate in DNA?
Replies: 1
Views: 1218

What is the use of etidium bromide in gels if dyes are used?

... bromide in agarose gels?Because from what i know, etidium bromide actually can be detected by autoradiography and shows fluorescence when they intercalate bands. But if bands are shown by the blue dye, what then is the use of etidium bromide?

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by appleguo
Wed Feb 01, 2006 7:55 am
 
Forum: Molecular Biology
Topic: What is the use of etidium bromide in gels if dyes are used?
Replies: 2
Views: 2023


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