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Of a living body or its parts: physically and functionally complete: having no relevant component removed or destroyed.

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Taqman probe design using FISH probe (need help)

... (but not so high as a fish probe) and will be hydrolysed by Taq DNA polymerase at every replication, thus releasing the fluorescence (quenched in intact probe) that will be measured to quantify the starting level of template. Therefore, you can design primers and probes so that they will fall ...

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by protoemics
Wed Feb 04, 2015 8:11 am
Forum: Bioinformatics
Topic: Taqman probe design using FISH probe (need help)
Replies: 2
Views: 586

Cannabis regeneration technique

... which you remove all the buds without cutting the branches, stems and leafs. You just trim the buds off the plant, leaving the rest of the plant intact. And leave the lights on a 12/12 cycle. What happens? Will the plant continue to produce new flowers? Is it really necessary to chop everything ...

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by Yolanda89
Sun Jul 27, 2014 5:21 pm
Forum: Botany Discussion
Topic: Cannabis regeneration technique
Replies: 0
Views: 2064

RNA integrity and RNA fragmentation?

... following experiments, I know that RNA quality so called RNA integrity is so important. So why there is a RNA fragmentation kit? Do we need RNA intact or fragmented?

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by golgicik
Mon Feb 03, 2014 9:15 pm
Forum: Molecular Biology
Topic: RNA integrity and RNA fragmentation?
Replies: 1
Views: 1343

Why does 28S rRNA appear as 4000 nt on a bioanalyzer

... on the internet showing Raji cells with a 28S/18S ratio of 2,7 and a 28S band size of 3900/4000 nt, so I figured the 28S rRNA would still be intact.) I can't find any explanation for this on the internet, so I hope someone here can help me out with this one. Thanks a lot in advance.

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by Nickythe100th
Sun Sep 08, 2013 9:05 am
Forum: Genetics
Topic: Why does 28S rRNA appear as 4000 nt on a bioanalyzer
Replies: 0
Views: 1256


... fine, plasmides will be taken up by competent cells and colonies will grow. Any comment on this? Will those nick be gone and will bacteria amplify intact circular dsDNA plasmids? tx

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by kk
Fri Jul 19, 2013 12:50 pm
Forum: Molecular Biology
Replies: 9
Views: 7132
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