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The act of furnishing with authoritative directions.

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Minimum Data Points for elisa standard curve

... and 1 negative standard (conc= 0 IU/ml).Is it practical to use 4PL or 5PL equations for only 5 data points( 5 standards)? 2. In Elisa test instruction that the company gave me, it is noted that the calibration curve should be a linear plot. But my results can be better explained by non ...

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by rgm
Sun Feb 01, 2015 7:19 am
Forum: Molecular Biology
Topic: Minimum Data Points for elisa standard curve
Replies: 0
Views: 327

Antibody Reconstitution

Hi All, I have question regarding some antibodies I wish to reconstitute. The instruction form that came with the order states, "Reconstitute with 0.1 ml sterile distilled water (Final concentration=1mg/ml. Final buffer = 0.01 M PBS, pH 7.4 with 10 mg/ml BSA ...

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by knclayton
Sun Feb 19, 2012 9:36 pm
Forum: Molecular Biology
Topic: Antibody Reconstitution
Replies: 1
Views: 1346

Re: How the soil knows the different seeds

... the seed. The seeds of a specific plant contain DNA that is unique to that plant. To put it simply, the DNA contained in the seed basically is an instruction manual for the plant, that tells it when to grow and how the cells should divide and differentiate. The soil is only a medium for the seed/plant ...

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by ehelms
Wed Nov 09, 2011 6:12 pm
Forum: General Discussion
Topic: How the soil knows the different seeds
Replies: 2
Views: 1223

GeneMarker basics - help!

Hi all, I am using GeneMarker for fragment analysis of microsatellites. I've read the instruction manual, but the whole procedure is completely new to me so many of the terms aren't explained fully enough for me to understand. Does anyone know of an online tutorial ...

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by lkay9
Tue Jun 21, 2011 4:14 pm
Forum: Molecular Biology
Topic: GeneMarker basics - help!
Replies: 0
Views: 1208

Re: Theories - Origin of Life

... rigid form with a specialized ability to transmit electrical signals etc etc. In the human genome there are about 200 different cell types.. This instruction set (genome) apparently does not contain all the instructions needed for cell growth. These instructions are extra or above (epi) to the ...

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by scottie
Sun Jun 19, 2011 10:55 am
Forum: Evolution
Topic: Theories - Origin of Life
Replies: 563
Views: 1135381
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