Dictionary » I » Insertion

Insertion

Insertion

1. (Science: anatomy) The place of attachment, as of a muscle to the bone which it moves.

2. (Science: genetics) a rare nonreciprocal translocation involving three breaks in which a segment is removed from one chromosome and then inserted into a broken region of a nonhomologous chromosome.

Origin: L. Inserere = to join to


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How

... of what you want to do. But technically if you just want to have 2genes one after the next to insert you could either plasmids with multiple insertion sites, make your construction in those (easy) then excise the double insert and transfer it to the genomic DNA as you would do. If you want ...

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by canalon
Sat Nov 02, 2013 6:15 pm
 
Forum: Molecular Biology
Topic: How
Replies: 4
Views: 903

Re: How

multiple gene insertion by conventional transformation is very time consuming and required selectable marker genes :( . So I have to do different..................? Do you have any idea ????? :(

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by Helics
Tue Oct 29, 2013 8:52 am
 
Forum: Molecular Biology
Topic: How
Replies: 4
Views: 903

Re: Locus specificity and ERVs - Q from a creationist

... are the major players and drivers of evolution. When they insert into host genome, they can alter host genes located at and around the insertion point - they can disrupt genes, activate genes, alter gene expression.

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by Cat
Sun Sep 15, 2013 4:08 pm
 
Forum: Genetics
Topic: Locus specificity and ERVs - Q from a creationist
Replies: 4
Views: 1136

Inserting cDNA into a retroviral vector?..

you will hardly insert dsDNA into ssRNA. You have to make RNA from it and that use for the insertion. But you have to be aware whether your retrovirus contains + or - RNA strand.

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by JackBean
Mon Mar 11, 2013 12:20 am
 
Forum: Genetics
Topic: Inserting cDNA into a retroviral vector?..
Replies: 3
Views: 2149

RNA

I think the answer to question 2 is formation of chimeric proteins because insertion, deletion and substitution are possible consequences of RNA splicing.

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by revision
Mon Nov 26, 2012 12:01 pm
 
Forum: Molecular Biology
Topic: RNA
Replies: 6
Views: 7206
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