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Inserted

Inserted

Attached to, arising from.


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Help: Confused with primers in a PCR of a vector?

1) positive control (T7-gene-T3). 2) if your gene is inserted into the vector so the beginning of the gene is right next to T7: T7-gene. If your gene is inserted into the vector so that the beginning of the gene is next to T3: Nothing since both primers ...

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by poobear
Fri Apr 11, 2014 9:58 pm
 
Forum: Molecular Biology
Topic: Help: Confused with primers in a PCR of a vector?
Replies: 2
Views: 2429

Problem with the normalization of PCR Ct values

... When dealing with exponents, division corresponds to subtraction... So, I will subtract the Ct values to get the ΔCt. These values are inserted to the statistical package. After the statistical analysis is finished, I have to interpret them... Provided that ΔCt=Ct reference - Ct target=X, ...

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by Tzia
Wed May 01, 2013 8:48 pm
 
Forum: Molecular Biology
Topic: Problem with the normalization of PCR Ct values
Replies: 5
Views: 3668

Re: question for evolutionary biologist.

... species? They took the DNA from one species, added some non-functional "comment lines", removed the DNA from a closely related species, inserted the DNA-with-comment-lines into it, and the new cell was viable, functionally the same as the cell it came from. You are not accurate in your ...

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by scottie
Thu Sep 13, 2012 1:22 pm
 
Forum: Evolution
Topic: question for evolutionary biologist.
Replies: 26
Views: 27785

Scientific paper on DNA replication & gel electrophoresis

... pyrimidine dimer (CPD) in leading strand of DNA -They constructed a plasmid containing oriC and a single CPD in the leading-strand -They inserted two terB sites to get a one unidirectional replication system -Only the minimal protein required to sustain oriC and replication were used. ...

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by dekk1525
Sat Sep 08, 2012 11:17 pm
 
Forum: Molecular Biology
Topic: Scientific paper on DNA replication & gel electrophoresis
Replies: 0
Views: 4796

Re: question for evolutionary biologist.

... species? They took the DNA from one species, added some non-functional "comment lines", removed the DNA from a closely related species, inserted the DNA-with-comment-lines into it, and the new cell was viable, functionally the same as the cell it came from. If the original cell has the ...

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by Rap
Wed Aug 29, 2012 8:09 pm
 
Forum: Evolution
Topic: question for evolutionary biologist.
Replies: 26
Views: 27785
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