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Insert

Insert

1. An additional length of base pairs in dna that has been introduced into that dna.

2. An additional length of bases that has been introduced into rna.

3. An additional length of amino acids that has been introduced into a protein.


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Help: Confused with primers in a PCR of a vector?

It's basically designed so that you see both length of your insert and it's direction. Did you use blunt-end cloning? Because otherwise one doesn't need to be so careful about the directions.

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by JackBean
Sun May 04, 2014 12:00 pm
 
Forum: Molecular Biology
Topic: Help: Confused with primers in a PCR of a vector?
Replies: 2
Views: 2226

Help: Confused with primers in a PCR of a vector?

... chunk of DNA? 3) coding region - T7 4) T3 - coding region I think the point of this entire process was to just determine the directionality of our insert. Because later in the lab, we did a whole bunch of sequence alignments with our gene (of both strands) and BLASTed it. But...I am just not really ...

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by keetner
Sat Mar 15, 2014 3:21 am
 
Forum: Molecular Biology
Topic: Help: Confused with primers in a PCR of a vector?
Replies: 2
Views: 2226

Fossil rabbits in the Precambrian!

... seems to be, a fossil can't exist before it's era. Can't find a fossil out of order. My question is, why not? If you use the extreme example of *insert animal* in the Precambrian, this makes sense. Otherwise, I don't quite get it. For example, we have found animals living in eras far before the ...

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by Steiner
Sun Dec 22, 2013 8:25 pm
 
Forum: Evolution
Topic: Fossil rabbits in the Precambrian!
Replies: 0
Views: 1733

Re: Re:

... although you may require some help from a medical professional with interpreting them -- for a cost of twenty to thirty bucks or euros or quid or [insert the equivalent in your currency here]. Unfortunately those tests do not normally measure other clotting factors than thrombocytes. One useful ...

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by snowathlete
Fri Dec 13, 2013 2:08 pm
 
Forum: Microbiology
Topic: What do you think these are, in my blood?
Replies: 16
Views: 8791

Re: Re:

... although you may require some help from a medical professional with interpreting them -- for a cost of twenty to thirty bucks or euros or quid or [insert the equivalent in your currency here]. Unfortunately those tests do not normally measure other clotting factors than thrombocytes. One useful ...

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by biohazard
Fri Dec 13, 2013 8:13 am
 
Forum: Microbiology
Topic: What do you think these are, in my blood?
Replies: 16
Views: 8791
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