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Dictionary » I » Inoculum

Inoculum

Definition

noun, plural

(1) Cells used in an inoculation, such as cells added to start a culture.

(2) A biological material (like a virus or toxin or immune serum) that is injected into a human to induce or increase immunity to a particular disease.


Supplement

Word origin: New Latin, from Latin inoculāre, to graft a scion.

Synonym: inoculant.
See also: inoculation.


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Re: Sterlization by Bleach, Water Purification Tablets

... do suggest you read the literature on this subject. Of course the presence of chlorine wiil affect growth but it's also important to consider the inoculum you'll use and what protcol for recovery. Some bugs grow well in distiled water approahing milion per ml in a relatively short time whereas ...

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by JorgeLobo
Sat Jan 28, 2012 2:28 pm
 
Forum: Microbiology
Topic: Sterlization by Bleach, Water Purification Tablets
Replies: 3
Views: 2457

Incubation of Soil Fungi

... without cytokinesis (cell division). One or a 1000 distinct fungal cells can give 1 cfu. Similarly serial dilution is invalid procedure to reduce inoculum concentration. You could try harvesting and resuspending conidiospores but precise measurment even with these is not readily obtained and multicellular ...

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by JorgeLobo
Sat Nov 05, 2011 12:52 am
 
Forum: Microbiology
Topic: Incubation of Soil Fungi
Replies: 4
Views: 1867

Baculovirus amplification in sf9 cells.

... 10e8(seeing light infection and less if you don´t see ) prepare a p2 in the same way in a B75flask with 1.5x10e7 cells, and after 4 days prepare inoculum for p3. at MOI0.2, after four days collect your p3(This is able to do repeated infections for protein production) and it would be better if ...

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by protold
Tue Jun 28, 2011 5:39 am
 
Forum: Cell Biology
Topic: Baculovirus amplification in sf9 cells.
Replies: 1
Views: 1773

Re: incubation of fungi

It would help if you'd add some details - identify the specimen that serves as inoculum and the medium you're using. Generally and as biohazard said, incubation conventionally is at a lower temp for fungi but rarely do medical labs incubate 2 to 4 weeks. If you're ...

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by JorgeLobo
Mon Feb 16, 2009 1:59 pm
 
Forum: Microbiology
Topic: incubation of fungi
Replies: 25
Views: 16327

Re: gel electrophoresis & assays help please

... working concentration with and without IPTG (final concentration of 1mM). Recommend cultures and inoculums for the practical are as follows: Inoculum Antibiotic Supplement 1 pSP1 Kan IPTG 2 pSP1 Kan 3 Red Colony Amp IPTG 4 Red Colony Amp 5 Large Clearing Zone Amp IPTG 6 Large Clearing Zone ...

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by RachBio
Sun Feb 08, 2009 9:26 pm
 
Forum: Molecular Biology
Topic: gel electrophoresis & assays help please
Replies: 6
Views: 6397
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