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Inoculum

Definition

noun, plural

(1) Cells used in an inoculation, such as cells added to start a culture.

(2) A biological material (like a virus or toxin or immune serum) that is injected into a human to induce or increase immunity to a particular disease.


Supplement

Word origin: New Latin, from Latin inoculāre, to graft a scion.

Synonym: inoculant.
See also: inoculation.


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Re: blank and substrate control give OD reads

... and controls in more detail including some example absorbances so we can make better idea what you have in there? detail method as below; 1) grow inoculum of xylanase variants in 96 multiwell plate, overnight, 37oC 2) expression in deep well plate for 24hrs, 30oC by 5% inoculation 3) centrifuge ...

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by intandiana
Sun Jan 19, 2014 5:57 am
 
Forum: Molecular Biology
Topic: blank and substrate control give OD reads
Replies: 9
Views: 3400

indole test reaction

... indole test, since the Techical Data Sheet of LTB says it can be used for such biochemical test ( it has Typtose), i incubated the 5 tubes (with inoculum) at 44.5 degrees and after 24 hrs. i dropped kovacs reagent to the tubes, after a while 1 tube has a red ring while the other tubes has green ...

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by magandangbutiki
Fri Aug 02, 2013 7:53 am
 
Forum: Microbiology
Topic: indole test reaction
Replies: 0
Views: 703

Re: Sterlization by Bleach, Water Purification Tablets

... do suggest you read the literature on this subject. Of course the presence of chlorine wiil affect growth but it's also important to consider the inoculum you'll use and what protcol for recovery. Some bugs grow well in distiled water approahing milion per ml in a relatively short time whereas ...

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by JorgeLobo
Sat Jan 28, 2012 2:28 pm
 
Forum: Microbiology
Topic: Sterlization by Bleach, Water Purification Tablets
Replies: 3
Views: 3324

Incubation of Soil Fungi

... without cytokinesis (cell division). One or a 1000 distinct fungal cells can give 1 cfu. Similarly serial dilution is invalid procedure to reduce inoculum concentration. You could try harvesting and resuspending conidiospores but precise measurment even with these is not readily obtained and multicellular ...

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by JorgeLobo
Sat Nov 05, 2011 12:52 am
 
Forum: Microbiology
Topic: Incubation of Soil Fungi
Replies: 4
Views: 3089

Baculovirus amplification in sf9 cells.

... 10e8(seeing light infection and less if you don´t see ) prepare a p2 in the same way in a B75flask with 1.5x10e7 cells, and after 4 days prepare inoculum for p3. at MOI0.2, after four days collect your p3(This is able to do repeated infections for protein production) and it would be better if ...

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by protold
Tue Jun 28, 2011 5:39 am
 
Forum: Cell Biology
Topic: Baculovirus amplification in sf9 cells.
Replies: 1
Views: 2893
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