Dictionary » I » Inoculation



noun, plural: inoculations


The process of introducing an antigenic substance or vaccine into the body to trigger immune response against a specific disease.


The act of introducing microorganism or suspension of microorganisms (e.g. bacteria) into a culture medium.


Inoculation is the act or process of introducing something to where it will grow or reproduce. It is most commonly used in respect of the introduction of a serum, vaccine, or antigenic substance into the body of a human or animal, especially to produce or boost immunity to a particular disease.

It may also refer to the communication of a disease to a living organism by transferring its causative agent into the organism, to implant microorganisms or infectious material into a culture medium.

Word origin: Origin: 1400–50; late ME < L inoculātiōn- (s. of inoculātiō), an engrafting.

Related forms: inoculate (verb), inoculum (noun).
Related phrases: inoculation loop.

See also: vaccination.

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Re: blank and substrate control give OD reads

... as below; 1) grow inoculum of xylanase variants in 96 multiwell plate, overnight, 37oC 2) expression in deep well plate for 24hrs, 30oC by 5% inoculation 3) centrifuge to harvest the enzyme 4) distribute substrate (pre-treated kenaf) equally to 96 deep well plate 5) add 250ul enzyme (from ...

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by intandiana
Sun Jan 19, 2014 5:57 am
Forum: Molecular Biology
Topic: blank and substrate control give OD reads
Replies: 9
Views: 6509

Help! "Unknown Bacteria" I have no idea where to look =T

... an answer. Your SIM is most likely non-motile but don't count it out I have been told to look for a "tornado" growth around immediate inoculation line or for a "hurricane" growth spread through out the media. Another test, although tricky to read, is the nitrate test. If ...

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by Smaguss
Tue Dec 04, 2012 5:00 am
Forum: Microbiology
Topic: Help! "Unknown Bacteria" I have no idea where to look =T
Replies: 1
Views: 4049

How does pH change during sporulation? - Help

... T = 0 to 8 hours, the pH decreased from about 7.4 to 6.8. We didn't measure it again until the next day (so a total of 28 hours passed initial inoculation) and the pH rose to 6.9. Then, another measurement was taken that day where it dropped to 6.8 again. According to my TA, this rise and drop ...

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by keetner
Mon Oct 22, 2012 10:22 pm
Forum: Microbiology
Topic: How does pH change during sporulation? - Help
Replies: 2
Views: 2080


No - you'd have to develop a test for indole in mixed culture of direct water inoculation and other bacteria produce indole (from tryptophane) including Vibrio, Pleisomonas, Salmonella, Citrobacter spp.

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by JorgeLobo
Tue Apr 24, 2012 2:37 am
Forum: General Discussion
Topic: indole
Replies: 5
Views: 2256

Re: Microbiology Investigation improvements?

... solution into the GSE section of the plate. 15. Place under the Bunsen burner to dry. 16. Label plates clearly with initials, E. coli and date of inoculation. 17. Incubate the plates at 37+/-1ºC. 18. Tidy away all equipment, sterilise all biohazards. Results % effectiveness determined by the amount ...

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by Squirrell
Mon Feb 20, 2012 8:30 am
Forum: General Discussion
Topic: Microbiology Investigation improvements?
Replies: 11
Views: 11259
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