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Inducer

An agent capable of activating specific genes.A molecule that inhibits the action of the repressor of an operon, preventing it from freely binding with the operator gene and disabling its function.


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Determine whether gene lac I and lac O is functioning?

My sample is treated in 3 ways: No inducer, with lactose , with IPTG. ONPG is used in all sample to determine the beta galactosidase. I know that both lactose and IPTG can associate with lac repressor and cause expression. If the result of ...

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by eos
Fri May 09, 2014 11:57 am
 
Forum: General Discussion
Topic: Determine whether gene lac I and lac O is functioning?
Replies: 0
Views: 497

Secondary Sugars(metabolism/sugar/bacteria/Lac Operon)

... that seemed to be of use; that secondary sugars might be sugars the require cAMP and CRP to be the effector of the operon(along with the operon inducer of course). Since gallactose is what the cell wants the most then the other metabolic operons(like lactose) should be off so any sugar that ...

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by Vulpess
Mon Dec 16, 2013 6:55 am
 
Forum: Microbiology
Topic: Secondary Sugars(metabolism/sugar/bacteria/Lac Operon)
Replies: 2
Views: 1656

Secondary Sugars(metabolism/sugar/bacteria/Lac Operon)

... cells prefer galactose, but will still use lactose, so does that mean lactose is a secondary sugar? Also does that make allolactose(lac operon inducer) a secondary sugar? Thanks

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by Vulpess
Mon Dec 16, 2013 3:40 am
 
Forum: Microbiology
Topic: Secondary Sugars(metabolism/sugar/bacteria/Lac Operon)
Replies: 2
Views: 1656

DIFFERENT BETWEEN REPRESSOR AND INDUCER

Activator and repressor bind DNA. Activator = transcription; repressor = no transcription (simplified). Inducer does NOT bind DNA. Inducer binds either activator OR repressor. It makes activator BETTER able to bind DNA = more transcription OR it makes repressor LESS able to ...

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by Cat
Fri Oct 19, 2012 12:15 am
 
Forum: Molecular Biology
Topic: DIFFERENT BETWEEN REPRESSOR AND INDUCER
Replies: 1
Views: 2680

DIFFERENT BETWEEN REPRESSOR AND INDUCER

CAN ANY BODY HELP ME TO UNDERSTAND BASIC DIFFERENT BETWEEN REPRESSOR AND INDUCER. AND WHAT IS THE DIFFERENT BETWEEN INDUCER AND ACTIVATOR PROTEIN IN RESPECT TO OPERONS ??

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by Helics
Wed Oct 17, 2012 3:23 pm
 
Forum: Molecular Biology
Topic: DIFFERENT BETWEEN REPRESSOR AND INDUCER
Replies: 1
Views: 2680
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