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Indirect assay

Indirect assay

For antibody; an application of the elisa method in which serum being tested for antibody is added to wells coated with known antigen; presence of antibody bound to the antigen coat can be determined by addition of immunoglobulin-specific antibody to which is linked the enzyme of the indicator system, followed by addition of substrate to the washed aggregate.


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Direct or indirect protein-DNA interaction

... leading to the gene of interest? All I have to work with is the regulatory region of the gene of interest. I'm trying to think of ways to assay this, but I can't come up with anything useful. Perhaps some kind of reporter gene assay might work? But reporter genes can't tell me what (if ...

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by nukeman
Wed Oct 31, 2012 5:21 pm
 
Forum: Molecular Biology
Topic: Direct or indirect protein-DNA interaction
Replies: 1
Views: 1016

Potential relationship between non-enzymatic antioxidants

... cell population, are known to synthesize testosterone in situ. Indirect evidence indicated that an oxytocin-like peptide was also present ... total glutathione were measured. Using the dichlorofluorescin (DCF-DA) assay, it was determined that isolated Leydig cells were capable of accumulating ...

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by ianjager
Thu Sep 18, 2008 5:08 pm
 
Forum: Cell Biology
Topic: Potential relationship between non-enzymatic antioxidants
Replies: 2
Views: 9169

The Fiber Disease

... signal amplification according to the manufacturer's instructions (TSA indirect, NEN Life Sciences, Boston, MA). Cy5-streptavidin (Amersham, Piscataway, ... the DRIPs clade. Development and Use of a Rhinosporidium-Specific PCR Assay A PCR assay specific for R. seeberi was developed. Primers were ...

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by al
Mon Dec 25, 2006 1:39 am
 
Forum: Human Biology
Topic: The Fiber Disease
Replies: 7403
Views: 5410412

The Fiber Disease

... T. C., 2003, “Measurement of hs-CRP by an Immunochemiluminonmetric Assay”, submitted to Clinic Chemistry. (in USA) (SCI) # Lin, S., Liu, C. ... and Ming-Chang Yang, “A Study on Monitoring of Vinyl Chloride Gas by Indirect Sensing Method”, J. Electrochem. Soc., 150, pp. H99-H104 (2003) ...

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by Nadas Moksha
Wed Dec 06, 2006 5:20 pm
 
Forum: Human Biology
Topic: The Fiber Disease
Replies: 7403
Views: 5410412

Methods for diet analysis

Nope ELISA means Enzyme linked Immuno-sorbent Assay. Basically you have a plate that fixes your sample then you add antibodies targeted at what you want to identify and marking (direct or indirect) to detct the fixation (or not) of your antibodies, The spectrophotometer ...

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by canalon
Sat Sep 30, 2006 3:51 am
 
Forum: Molecular Biology
Topic: Methods for diet analysis
Replies: 3
Views: 1478


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