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Incubation

Incubation

(Science: microbiology) The development of an infectious disease from the entrance of the pathogen to the appearance of clinical symptoms.

Origin: L. Incubatio


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Re: centrifugation of hemocytes

I can't see any pellet, BUT today it tried again, after 24h of incubation (cells have time to adhere), and centrifugation at 400 for 5 min, I gently took off the medium and resuspend the cells by pipetting thoroughly up/down: the viability worked well. ...

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by Thom
Tue Jan 28, 2014 4:16 pm
 
Forum: Cell Biology
Topic: centrifugation of hemocytes
Replies: 5
Views: 2597

blank and substrate control give OD reads

... well plate by adding substrate (kenaf) + buffer (50mM sodium acetate, pH 5.0) + enzyme solution and incubate for 4 hours in waterbath. after 4hrs incubation, the reaction is centrifuged to collect only reaction solution (without the substrate) then stop solution with DNS and incubate at 100oC, ...

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by intandiana
Wed Jan 15, 2014 7:21 am
 
Forum: Molecular Biology
Topic: blank and substrate control give OD reads
Replies: 9
Views: 3401

Re:

... be negative. It's either Oxidative, Fermentative, or both. "METHYL RED TEST – NEGATIIVE" - Did you re-test after additional 48HRS of incubation? Hey thanks for the reply I did further research and found out that... Gram reaction:+ Shape: cocci Acid-fast: - Spores: - Motility: - Grows ...

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by microbiologyhelp
Sun Dec 01, 2013 11:36 am
 
Forum: Microbiology
Topic: URGENT HELP IDENTIFYING UNKNOWN BACTERIA!!!
Replies: 6
Views: 5643

URGENT HELP IDENTIFYING UNKNOWN BACTERIA!!!

... be negative. It's either Oxidative, Fermentative, or both. "METHYL RED TEST – NEGATIIVE" - Did you re-test after additional 48HRS of incubation?

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by Cat
Fri Nov 29, 2013 5:17 pm
 
Forum: Microbiology
Topic: URGENT HELP IDENTIFYING UNKNOWN BACTERIA!!!
Replies: 6
Views: 5643

question about G418 sceening test

... cells (Huh-7). I start with a T25 flask of Huh-7 about 80% confluence, and passed it to a 6 well plate, each is about 15% confluence. After 24hr incubation with 2 mL regular DMEM, I changed to DMEM with G418 with different concentrations. The G418 disulfide 50mg/mL stock in H2O was from Sigma(G8168). ...

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by cavaliersun2003
Mon Nov 11, 2013 7:28 am
 
Forum: Cell Biology
Topic: question about G418 sceening test
Replies: 0
Views: 1029
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