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Incubation

Incubation

(Science: microbiology) The development of an infectious disease from the entrance of the pathogen to the appearance of clinical symptoms.

Origin: L. Incubatio


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Re:

... At near bottom there is no oxygen at all. If we add suitable nutrients in the agar and innoculate with a needle (stab innoculation) after suitable incubation time we can know microbe's oxygen requirements. Aerophile grow on the top, microaerophile in middle and anerobic at bottom. It will work ...

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by sosippus
Wed Sep 24, 2014 7:44 pm
 
Forum: Microbiology
Topic: Oxygen requirements of eukaryotes
Replies: 3
Views: 337

Oxygen requirements of eukaryotes

... At near bottom there is no oxygen at all. If we add suitable nutrients in the agar and innoculate with a needle (stab innoculation) after suitable incubation time we can know microbe's oxygen requirements. Aerophile grow on the top, microaerophile in middle and anerobic at bottom. It will work ...

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by josem
Wed Sep 24, 2014 5:28 pm
 
Forum: Microbiology
Topic: Oxygen requirements of eukaryotes
Replies: 3
Views: 337

Re: centrifugation of hemocytes

I can't see any pellet, BUT today it tried again, after 24h of incubation (cells have time to adhere), and centrifugation at 400 for 5 min, I gently took off the medium and resuspend the cells by pipetting thoroughly up/down: the viability worked well. ...

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by Thom
Tue Jan 28, 2014 4:16 pm
 
Forum: Cell Biology
Topic: centrifugation of hemocytes
Replies: 5
Views: 2914

blank and substrate control give OD reads

... well plate by adding substrate (kenaf) + buffer (50mM sodium acetate, pH 5.0) + enzyme solution and incubate for 4 hours in waterbath. after 4hrs incubation, the reaction is centrifuged to collect only reaction solution (without the substrate) then stop solution with DNS and incubate at 100oC, ...

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by intandiana
Wed Jan 15, 2014 7:21 am
 
Forum: Molecular Biology
Topic: blank and substrate control give OD reads
Replies: 9
Views: 3707

Re:

... be negative. It's either Oxidative, Fermentative, or both. "METHYL RED TEST – NEGATIIVE" - Did you re-test after additional 48HRS of incubation? Hey thanks for the reply I did further research and found out that... Gram reaction:+ Shape: cocci Acid-fast: - Spores: - Motility: - Grows ...

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by microbiologyhelp
Sun Dec 01, 2013 11:36 am
 
Forum: Microbiology
Topic: URGENT HELP IDENTIFYING UNKNOWN BACTERIA!!!
Replies: 7
Views: 6185
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