Incubation

... the fungi then how you have maintained it? Anyway, normally for fungal growth and maintenance you need to grow the fungi at 26-28C temparature for incubation on Potato Dextrose Agar (PDA). While you carry out with some experiments with your fungi, you need to grow them on Sabouraud Dextrose Agar ...

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... seems to be solved. Not sure which step went wrong but the second signal began to appear when I diluted the second antibody 2X more and used less incubation time for the TSA reaction (about 3 min).

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... chloride procedure. To test them, i plated them out on agar plate containing: Kanamycin, Tetracyclon, Ampicilin an control. After overnight incubation, there is bacteria colonies in all plates. These colonies are so dense we cant not see them separately. Since I have kept my original stock ...

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... cells to the detergent for longer time 3. Increase the concentration of the nuclease 4. Decrease the concentration of the nuclease 5. Increase the incubation time with the nuclease. 6. Increase the number of cells used I've put down 1,2,3,5 Thank u!

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... but I'm not a cellular biologist and I need some help. I isolated PBMC from the whole blood and then I seeded them in a 96 well plate. After incubation of 24h I would like to monitor the presence of a certain protein on their surface using a labelled antibody. Do I need to treat them as adherent ...

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