Dictionary » I » Incorporation

Incorporation

Incorporation

1. (Science: chemistry) The union of one substance with another or with others, in a composite mass.

2. (Science: psychology) a primitive unconscious defense mechanism in which aspects of another person are assimilated into the self through a figurative process of symbolic oral ingestion.


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Re: microsatellite instability

OK, another question: I can see how insertion/deletion can be detected by fragment analysis, but incorporation of a wrong nucleotide wouldn't cause change in the length of amplicon, so can that also be detected by fragment analysis?

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by kk
Thu Jul 10, 2014 2:45 pm
 
Forum: Molecular Biology
Topic: microsatellite instability
Replies: 3
Views: 2176

Re: Codons are triplets, but what's a Singlet, Doublet, etc?

... code and catalyzing their own formation from polynucleotide templates. These proteins are assignment catalysts capable of modulating the rate of incorporation of different amino acids at the position of different codons. The starting point of the model is a polynucleotide based polypeptide construction ...

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by GaryGaulin
Thu Oct 25, 2012 4:28 am
 
Forum: Bioinformatics
Topic: Codons are triplets, but what's a Singlet, Doublet, etc?
Replies: 4
Views: 11030

Mastermix in PCR

... the concentration of MgCl2. The higher the concentration of the MgCl2, the more active the enzyme is. As the concentration of MgCl2 increases, the incorporation of dNTPs increases. However, it also increases the the error rate - the rate at which an incorrect dNTP is incorporated into the resulting ...

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by daddyjames
Sun Oct 14, 2012 3:43 pm
 
Forum: Bioinformatics
Topic: Mastermix in PCR
Replies: 4
Views: 3254

Do the simplest bacteria have ribosomes and helicase?

Daemach, you must check chemical reactions in RNA synthesis before asking any more questions. Here's a link with a pic of actual nucleotide incorporation into oligo chain: http://sandwalk.blogspot.com/2007/03/how-rna-polymerase-works-chemical.html Thing is, A, C, G and T/U in DNA/RNA molecule ...

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by dustman
Sat May 26, 2012 11:04 pm
 
Forum: Molecular Biology
Topic: Do the simplest bacteria have ribosomes and helicase?
Replies: 27
Views: 13354

Re: how to add a "A" at PCR product ends

... add ONLY buffer, template, dATP, and Taq. Then it's absolutely impossible for Taq to add non-A bases on the ends. Even if your efficiency is low, incorporation of the A will occur at a MUCH higher rate than if you used raw PCR product containing all 4 trinucleotides.

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by CarmeanC
Fri Mar 09, 2012 6:38 pm
 
Forum: Molecular Biology
Topic: how to add a "A" at PCR product ends
Replies: 6
Views: 19341
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