Dictionary » I » Incorporation

Incorporation

Incorporation

1. (Science: chemistry) The union of one substance with another or with others, in a composite mass.

2. (Science: psychology) a primitive unconscious defense mechanism in which aspects of another person are assimilated into the self through a figurative process of symbolic oral ingestion.


Please contribute to this project, if you have more information about this term feel free to edit this page



Results from our forum


Re: Codons are triplets, but what's a Singlet, Doublet, etc?

... code and catalyzing their own formation from polynucleotide templates. These proteins are assignment catalysts capable of modulating the rate of incorporation of different amino acids at the position of different codons. The starting point of the model is a polynucleotide based polypeptide construction ...

See entire post
by GaryGaulin
Thu Oct 25, 2012 4:28 am
 
Forum: Bioinformatics
Topic: Codons are triplets, but what's a Singlet, Doublet, etc?
Replies: 4
Views: 9065

Mastermix in PCR

... the concentration of MgCl2. The higher the concentration of the MgCl2, the more active the enzyme is. As the concentration of MgCl2 increases, the incorporation of dNTPs increases. However, it also increases the the error rate - the rate at which an incorrect dNTP is incorporated into the resulting ...

See entire post
by daddyjames
Sun Oct 14, 2012 3:43 pm
 
Forum: Bioinformatics
Topic: Mastermix in PCR
Replies: 4
Views: 2605

Do the simplest bacteria have ribosomes and helicase?

Daemach, you must check chemical reactions in RNA synthesis before asking any more questions. Here's a link with a pic of actual nucleotide incorporation into oligo chain: http://sandwalk.blogspot.com/2007/03/how-rna-polymerase-works-chemical.html Thing is, A, C, G and T/U in DNA/RNA molecule ...

See entire post
by dustman
Sat May 26, 2012 11:04 pm
 
Forum: Molecular Biology
Topic: Do the simplest bacteria have ribosomes and helicase?
Replies: 27
Views: 11425

Re: how to add a "A" at PCR product ends

... add ONLY buffer, template, dATP, and Taq. Then it's absolutely impossible for Taq to add non-A bases on the ends. Even if your efficiency is low, incorporation of the A will occur at a MUCH higher rate than if you used raw PCR product containing all 4 trinucleotides.

See entire post
by CarmeanC
Fri Mar 09, 2012 6:38 pm
 
Forum: Molecular Biology
Topic: how to add a "A" at PCR product ends
Replies: 6
Views: 17883

Why RT-PCR

yep, your gene may be silenced after incorporation or your promoter may not work properly, thus you need to check expression of your transgene.

See entire post
by JackBean
Mon Jan 30, 2012 9:46 am
 
Forum: Molecular Biology
Topic: Why RT-PCR
Replies: 2
Views: 814
View all matching forum results

This page was last modified 21:16, 3 October 2005. This page has been accessed 3,037 times. 
What links here | Related changes | Permanent link