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Incomplete antibody

Incomplete antibody --> univalent antibody

An incomplete form of antibody that may coat antigen, but which according to the lattice theory does not have a second receptor for attachment to another molecule of antigen; in the case of Rh_ erythrocytes, such an anti-Rh antibody may coat the cells but not cause them to agglutinate in saline; however, agglutination does occur when such coated cells are suspended in serum or other protein media, such as albumin, therefore called serum agglutinin.

Synonym: incomplete antibody, inhibiting antibody.

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incomplete transfer of protein samples for western blot

... protein is small, around 18kd. I probed the membrane with my target antibody and saw absolutely nothing, the film didn't even show the outlines ... I see most of my protein was left on the gel it seems I am getting an incomplete transfer. However, on the other hand could it be the my protein ...

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by Newbie
Tue Oct 03, 2006 6:36 pm
Forum: Molecular Biology
Topic: incomplete transfer of protein samples for western blot
Replies: 0
Views: 1200


... vitro. This method is, however, dependent on the use of a high-quality antibody directed against a desired protein. So you must be able to produce ... time. Rest the rabbit for one month, then repeat the process using an incomplete adjuvant. You can expect to see good antibody titers about 10 ...

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by Dr.Stein
Mon Nov 28, 2005 9:02 am
Forum: Molecular Biology
Replies: 3
Views: 4677

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