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Inclusion bodies

Inclusion bodies

(Science: cell biology) nuclear or cytoplasmic structures with characteristic staining properties, usually found at the site of virus multiplication. Semi crystalline arrays of virions, capsids or other viral components.


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Re: Re:

... rods that are difficult to tell apart from some other causes, such as inclusion bodies or precipitates in the staining solution. The good side, though, is that Bartonella ...

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by biohazard
Tue Dec 10, 2013 2:02 pm
 
Forum: Microbiology
Topic: What do you think these are, in my blood?
Replies: 16
Views: 8757

Removing MBP tag

if is your protein in inclusion bodies, you express it in E. coli, right? What about trying yeast or something else? If you have problems ...

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by JackBean
Thu Oct 27, 2011 10:18 am
 
Forum: Molecular Biology
Topic: Removing MBP tag
Replies: 12
Views: 8560

Re: Removing MBP tag

... several chromatographic steps The untagged protein is mostly found in inclusion bodies so it would be difficult to purify it. Anyway it might be worth trying it. The cells ...

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by Papaver
Thu Oct 27, 2011 9:59 am
 
Forum: Molecular Biology
Topic: Removing MBP tag
Replies: 12
Views: 8560

restriction enzyme question.

... solutions. Thus the hydrophobic proteins would aggregate and form inclusion bodies.

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by JackBean
Sat Jun 18, 2011 10:26 am
 
Forum: General Discussion
Topic: restriction enzyme question.
Replies: 9
Views: 4415

Re:

... degraded? Are you sure, it is produced at all? That it's not put into inclusion bodies? Do you have correct activity measurement (including reall substrates etc.)? thanks ...

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by wangzhy
Sat Nov 14, 2009 4:01 pm
 
Forum: General Discussion
Topic: how to decrease the degradation of protein in purificaton
Replies: 3
Views: 1721
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