Dictionary » I » Inclusion

Inclusion

Inclusion

1. The act of including, or the state of being included; limitation; restriction; as, the lines of inclusion of his policy.

2. (Science: chemical) a foreign substance, either liquid or solid, usually of minute size, inclosed in the mass of a mineral.

Origin: L. Inclusio: cf. F. Inclusion. See Include.


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Re: Re:

... special stains most bacteria simply look like various kinds of dots or rods that are difficult to tell apart from some other causes, such as inclusion bodies or precipitates in the staining solution. The good side, though, is that Bartonella species are normally susceptible to many common ...

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by biohazard
Tue Dec 10, 2013 2:02 pm
 
Forum: Microbiology
Topic: What do you think these are, in my blood?
Replies: 16
Views: 8760

reptile eggs

I think the inclusion of "calcified" was a mistake.

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by Darby
Thu Nov 03, 2011 11:56 pm
 
Forum: Zoology Discussion
Topic: reptile eggs
Replies: 4
Views: 2430

pEGFP-C1

... the plasmid? It could be a question of your PCR or your transfection. I suspect that it is your PCR because normally if there was at least partial inclusion there would be some level of expression. Why are you just cloning in GFP alone not linked to a protein? Can you elaborate on the steps that ...

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by daniel.kurz
Fri Oct 28, 2011 5:53 am
 
Forum: Molecular Biology
Topic: pEGFP-C1
Replies: 6
Views: 2799

Removing MBP tag

if is your protein in inclusion bodies, you express it in E. coli, right? What about trying yeast or something else? If you have problems with inclusion bodies and want to keep E.coli, I suggest decreasing incubation temperature to ~25°C ...

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by JackBean
Thu Oct 27, 2011 10:18 am
 
Forum: Molecular Biology
Topic: Removing MBP tag
Replies: 12
Views: 8561

Re: Removing MBP tag

... would be easier to produce your protein with no tag and just purify through several chromatographic steps The untagged protein is mostly found in inclusion bodies so it would be difficult to purify it. Anyway it might be worth trying it. The cells that you are using to express the protein may ...

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by Papaver
Thu Oct 27, 2011 9:59 am
 
Forum: Molecular Biology
Topic: Removing MBP tag
Replies: 12
Views: 8561
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